Same substrate, many reactions: oxygen activation in flavoenzymes

E Romero, JR Gómez Castellanos, G Gadda… - Chemical …, 2018 - ACS Publications
Over time, organisms have evolved strategies to cope with the abundance of dioxygen on
Earth. Oxygen-utilizing enzymes tightly control the reactions involving O2 mostly by …

Discovery and characterization of a 5-hydroxymethylfurfural oxidase from Methylovorus sp. strain MP688

WP Dijkman, MW Fraaije - Applied and environmental …, 2014 - Am Soc Microbiol
In the search for useful and renewable chemical building blocks, 5-hydroxymethylfurfural
(HMF) has emerged as a very promising candidate, as it can be prepared from sugars. HMF …

Structure-based enzyme tailoring of 5-hydroxymethylfurfural oxidase

WP Dijkman, C Binda, MW Fraaije, A Mattevi - ACs Catalysis, 2015 - ACS Publications
5-Hydroxymethylfurfural oxidase (HMFO) is a flavin-dependent enzyme that catalyzes the
oxidation of many aldehydes, primary alcohols, and thiols. The three-step conversion of 5 …

Choline oxidases

G Gadda - The Enzymes, 2020 - Elsevier
Choline oxidase catalyzes the four-electron, two-step, flavin-mediated oxidation of choline to
glycine betaine. The enzyme is important both for medical and biotechnological reasons …

A High‐Performance Base‐Metal Approach for the Oxidative Esterification of 5‐Hydroxymethylfurfural

Y Sun, H Ma, X Jia, J Ma, Y Luo, J Gao, J Xu - ChemCatChem, 2016 - Wiley Online Library
Exploring high‐performance base‐metal approaches for the sustainable production of
chemicals from biomass is presently attracting immense interest and is truly important to …

Crystal Structure of Alcohol Oxidase from Pichia pastoris

C Koch, P Neumann, O Valerius, I Feussner, R Ficner - PLoS One, 2016 - journals.plos.org
FAD-dependent alcohol oxidases (AOX) are key enzymes of methylotrophic organisms that
can utilize lower primary alcohols as sole source of carbon and energy. Here we report the …

Metal-Triggered FAD Reduction in d-2-Hydroxyglutarate Dehydrogenase from Pseudomonas aeruginosa PAO1

JA Quaye, G Gadda - ACS Bio & Med Chem Au, 2024 - ACS Publications
Alcohol oxidation is an indispensable chemical reaction in biological systems. This process,
biologically catalyzed by alcohol dehydrogenases (ADHs) and alcohol oxidases (AOXs) …

Anaerobic methods for the transient-state study of flavoproteins: the use of specialized glassware to define the concentration of dioxygen

GR Moran - Methods in enzymology, 2019 - Elsevier
The methods described here concern the use specialized glassware to achieve accurate
sub-micromolar to low-millimolar dioxygen concentrations specifically for the study of …

The Pseudomonas aeruginosa PAO1 metallo flavoprotein D-2-hydroxyglutarate dehydrogenase requires Zn2+ for substrate orientation and activation

JA Quaye, G Gadda - Journal of Biological Chemistry, 2023 - ASBMB
Pseudomonas aeruginosa PAO1 d-2-hydroxyglutarate (D2HG) dehydrogenase
(PaD2HGDH) oxidizes D2HG to 2-ketoglutarate during the vital l-serine biosynthesis and is …

[HTML][HTML] Enantioselective oxidation of secondary alcohols by the flavoprotein alcohol oxidase from Phanerochaete chrysosporium

G Tjallinks, C Martin, MW Fraaije - Archives of Biochemistry and Biophysics, 2021 - Elsevier
The enantioselective oxidation of secondary alcohols represents a valuable approach for
the synthesis of optically pure compounds. Flavoprotein oxidases can catalyse such …