Gene drives gaining speed

E Bier - Nature Reviews Genetics, 2022 - nature.com
Gene drives are selfish genetic elements that are transmitted to progeny at super-Mendelian
(> 50%) frequencies. Recently developed CRISPR–Cas9-based gene-drive systems are …

CRISPR-Cas-based antimicrobials: design, challenges, and bacterial mechanisms of resistance

A Mayorga-Ramos, J Zúñiga-Miranda… - ACS Infectious …, 2023 - ACS Publications
The emergence of antibiotic-resistant bacterial strains is a source of public health concern
across the globe. As the discovery of new conventional antibiotics has stalled significantly …

CRISPR RNA-guided integrases for high-efficiency, multiplexed bacterial genome engineering

PLH Vo, C Ronda, SE Klompe, EE Chen, C Acree… - Nature …, 2021 - nature.com
Existing technologies for site-specific integration of kilobase-sized DNA sequences in
bacteria are limited by low efficiency, a reliance on recombination, the need for multiple …

Synthetic microbiology in sustainability applications

EM Jones, JP Marken, PA Silver - Nature Reviews Microbiology, 2024 - nature.com
Microorganisms are a promising means to address many societal sustainability challenges
owing to their ability to thrive in diverse environments and interface with the microscale …

Active genetic neutralizing elements for halting or deleting gene drives

XRS Xu, EA Bulger, VM Gantz, C Klanseck, SR Heimler… - Molecular cell, 2020 - cell.com
CRISPR-Cas9-based gene drive systems possess the inherent capacity to spread
progressively throughout target populations. Here we describe two self-copying (or active) …

Cancer cells employ an evolutionarily conserved polyploidization program to resist therapy

KJ Pienta, EU Hammarlund, RH Austin… - Seminars in cancer …, 2022 - Elsevier
Unusually large cancer cells with abnormal nuclei have been documented in the cancer
literature since 1858. For more than 100 years, they have been generally disregarded as …

CRISPR-based antimicrobials to obstruct antibiotic-resistant and pathogenic bacteria

D Palacios Araya, KL Palmer, BA Duerkop - PLoS Pathogens, 2021 - journals.plos.org
The rapid emergence of antibiotic-resistant bacteria and the comparatively limited
development of new antibacterial molecules pose a major threat to modern medicine by …

Assessment of a split homing based gene drive for efficient knockout of multiple genes

NP Kandul, J Liu, A Buchman, VM Gantz… - G3: Genes …, 2020 - academic.oup.com
Homing based gene drives (HGD) possess the potential to spread linked cargo genes into
natural populations and are poised to revolutionize population control of animals. Given that …

Environmental Galenics: large-scale fortification of extant microbiomes with engineered bioremediation agents

V de Lorenzo - … Transactions of the Royal Society B, 2022 - royalsocietypublishing.org
Contemporary synthetic biology-based biotechnologies are generating tools and strategies
for reprogramming genomes for specific purposes, including improvement and/or creation of …

Incorporating ecology into gene drive modelling

J Kim, KD Harris, IK Kim, S Shemesh… - Ecology …, 2023 - Wiley Online Library
Gene drive technology, in which fast‐spreading engineered drive alleles are introduced into
wild populations, represents a promising new tool in the fight against vector‐borne diseases …