Paving the way to single-molecule protein sequencing

L Restrepo-Pérez, C Joo, C Dekker - Nature nanotechnology, 2018 - nature.com
Proteins are major building blocks of life. The protein content of a cell and an organism
provides key information for the understanding of biological processes and disease. Despite …

Recent progress in solid‐state nanopores

K Lee, KB Park, HJ Kim, JS Yu, H Chae… - Advanced …, 2018 - Wiley Online Library
The solid‐state nanopore has attracted much attention as a next‐generation DNA
sequencing tool or a single‐molecule biosensor platform with its high sensitivity of …

Electro-osmotic capture and ionic discrimination of peptide and protein biomarkers with FraC nanopores

G Huang, K Willems, M Soskine, C Wloka… - Nature …, 2017 - nature.com
Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and
long read-length DNA sequencing applications. The analysis and sequencing of proteins …

Identification of single amino acid differences in uniformly charged homopolymeric peptides with aerolysin nanopore

F Piguet, H Ouldali, M Pastoriza-Gallego… - Nature …, 2018 - nature.com
There are still unmet needs in finding new technologies for biomedical diagnostic and
industrial applications. A technology allowing the analysis of size and sequence of short …

Nanopore-based measurements of protein size, fluctuations, and conformational changes

P Waduge, R Hu, P Bandarkar, H Yamazaki… - ACS …, 2017 - ACS Publications
Proteins are structurally dynamic macromolecules, and it is challenging to quantify the
conformational properties of their native state in solution. Nanopores can be efficient tools to …

Single-molecule sensing using nanopores in two-dimensional transition metal carbide (MXene) membranes

M Mojtabavi, A VahidMohammadi, W Liang… - ACS …, 2019 - ACS Publications
Label-free nanopore technology for sequencing biopolymers such as DNA and RNA could
potentially replace existing methods if improvements in cost, speed, and accuracy are …

Nanopore sensing at ultra-low concentrations using single-molecule dielectrophoretic trapping

KJ Freedman, LM Otto, AP Ivanov, A Barik… - Nature …, 2016 - nature.com
Single-molecule techniques are being developed with the exciting prospect of
revolutionizing the healthcare industry by generating vast amounts of genetic and proteomic …

Single molecule multiplexed nanopore protein screening in human serum using aptamer modified DNA carriers

JYY Sze, AP Ivanov, AEG Cass, JB Edel - Nature communications, 2017 - nature.com
The capability to screen a range of proteins at the single-molecule level with enhanced
selectivity in biological fluids has been in part a driving force in developing future diagnostic …

Single-molecule biosensors: Recent advances and applications

N Akkilic, S Geschwindner, F Höök - Biosensors and Bioelectronics, 2020 - Elsevier
Single-molecule biosensors serve the unmet need for real time detection of individual
biological molecules in the molecular crowd with high specificity and accuracy, uncovering …

Membrane protein structure, function, and dynamics: a perspective from experiments and theory

Z Cournia, TW Allen, I Andricioaei, B Antonny… - The Journal of …, 2015 - Springer
Membrane proteins mediate processes that are fundamental for the flourishing of biological
cells. Membrane-embedded transporters move ions and larger solutes across membranes; …