Transcriptional regulation is achieved through combinatorial interactions between regulatory
elements in the human genome and a vast range of factors that modulate the recruitment …

The assembly of sequence-specific enhancer-binding transcription factors (TFs) at cis-
regulatory elements in the genome has long been regarded as the fundamental mechanism …

Transcription factors (TFs) regulate gene expression by binding to specific consensus motifs
within the local chromatin context. The mechanisms by which TFs navigate the nuclear …

Live-cell measurement of protein binding to chromatin allows probing cellular biochemistry
in physiological conditions, which are difficult to mimic in vitro. However, different studies …

Multicolor imaging based on genetically encoded fluorescent proteins (FPs) is a powerful
approach to study several dynamic processes in a live cell. We report a monomeric orange …

Genomic footprinting has emerged as an unbiased discovery method for transcription factor
(TF) occupancy at cognate DNA in vivo. A basic premise of footprinting is that sequence …

Inside prokaryotic cells, passive translational diffusion typically limits the rates with which
cytoplasmic proteins can reach their locations. Diffusion is thus fundamental to most cellular …

The glucocorticoid receptor (GR) is a bona fide ligand‐regulated transcription factor. Cloned
in the 80s, the GR has become one of the best‐studied and clinically most relevant members …

Key processes of biological condensates are diffusion and material exchange with their
environment. Experimentally, diffusive dynamics are typically probed via fluorescent labels …

FRAP (fluorescence recovery after photo bleaching) is a method for determining diffusion in
material science. In industrial applications such as medications, foods, Medtech, hygiene …