Next-generation data filtering in the genomics era
Genomic data are ubiquitous across disciplines, from agriculture to biodiversity, ecology,
evolution and human health. However, these datasets often contain noise or errors and are …
evolution and human health. However, these datasets often contain noise or errors and are …
Big data in conservation genomics: Boosting skills, hedging bets, and staying current in the field
RM Schweizer, N Saarman, KM Ramstad… - Journal of …, 2021 - academic.oup.com
A current challenge in the fields of evolutionary, ecological, and conservation genomics is
balancing production of large-scale datasets with additional training often required to handle …
balancing production of large-scale datasets with additional training often required to handle …
On the causes, consequences, and avoidance of PCR duplicates: towards a theory of library complexity
NC Rochette, AG Rivera‐Colón, J Walsh… - Molecular ecology …, 2023 - Wiley Online Library
Library preparation protocols for most sequencing technologies involve PCR amplification of
the template DNA, which open the possibility that a given template DNA molecule is …
the template DNA, which open the possibility that a given template DNA molecule is …
A GT‐seq panel for walleye (Sander vitreus) provides important insights for efficient development and implementation of amplicon panels in non‐model organisms
ML Bootsma, KM Gruenthal… - Molecular Ecology …, 2020 - Wiley Online Library
Targeted amplicon sequencing methods, such as genotyping‐in‐thousands by sequencing
(GT‐seq), facilitate rapid, accurate, and cost‐effective analysis of hundreds of genetic loci in …
(GT‐seq), facilitate rapid, accurate, and cost‐effective analysis of hundreds of genetic loci in …
Estimating and accounting for genotyping errors in RAD‐seq experiments
L Bresadola, V Link, CA Buerkle… - Molecular Ecology …, 2020 - Wiley Online Library
In non‐model organisms, evolutionary questions are frequently addressed using reduced
representation sequencing techniques due to their low cost, ease of use, and because they …
representation sequencing techniques due to their low cost, ease of use, and because they …
Divergence With Gene Flow and Contrasting Population Size Blur the Species Boundary in Cycas Sect. Asiorientales, as Inferred From Morphology and RAD-Seq …
The divergence process of incipient species is fascinating but elusive by incomplete lineage
sorting or gene flow. Species delimitation is also challenging among those morphologically …
sorting or gene flow. Species delimitation is also challenging among those morphologically …
Effective double‐digest RAD sequencing and genotyping despite large genome size
Obtaining informative data is the ambition of any genomic project, but in nonmodel species
with very large genomes, pursuing such a goal requires surmounting a series of analytical …
with very large genomes, pursuing such a goal requires surmounting a series of analytical …
Palau's warmest reefs harbor thermally tolerant corals that thrive across different habitats
Ocean warming is killing corals, but heat-tolerant populations exist; if protected, they could
replenish affected reefs naturally or through restoration. Palau's Rock Islands experience …
replenish affected reefs naturally or through restoration. Palau's Rock Islands experience …
Rapid morphological divergence following a human-mediated introduction: The role of drift and directional selection
Abstract Theory predicts that when populations are established by few individuals, random
founder effects can facilitate rapid phenotypic divergence even in the absence of selective …
founder effects can facilitate rapid phenotypic divergence even in the absence of selective …
A reference‐free approach to analyse RADseq data using standard next generation sequencing toolkits
R Heller, C Nursyifa, G Garcia‐Erill… - Molecular Ecology …, 2021 - Wiley Online Library
Genotyping‐by‐sequencing methods such as RADseq are popular for generating genomic
and population‐scale data sets from a diverse range of organisms. These often lack a …
and population‐scale data sets from a diverse range of organisms. These often lack a …