Targeted genome editing using artificial nucleases has the potential to accelerate basic
research as well as plant breeding by providing the means to modify genomes rapidly in a …

TALEN were the first easy-to-use genome editing technology and sparked the genome
editing revolution. Their application in multiple species brought targeted mutagenesis to the …

Enhancers can regulate the promoters of their target genes over very large genomic
distances. It is widely assumed that mechanisms of enhancer action involve the …

Background The use of homologous recombination to precisely modify plant genomes has
been challenging, due to the lack of efficient methods for delivering DNA repair templates to …

The relative ease, speed, and biological scope of clustered regularly interspaced short
palindromic repeats (CRISPR)/CRISPR-associated Protein9 (Cas9)-based reagents for …

Gene silencing is instrumental to interrogate gene function and holds promise for
therapeutic applications. Here, we repurpose the endogenous retroviruses' silencing …

Adoptive immunotherapy using autologous T cells endowed with chimeric antigen receptors
(CAR) has emerged as a powerful means of treating cancer. However, a limitation of this …

Major advances in genome editing have recently been made possible with the development
of the TALEN and CRISPR/Cas9 methods. The speed and ease of implementing these …

Although great progress has been made in the characterization of the off-target effects of
engineered nucleases, sensitive and unbiased genome-wide methods for the detection of …

Engineered nucleases enable the targeted alteration of nearly any gene in a wide range of
cell types and organisms. The newly-developed transcription activator-like effector …