A growing body of research is investigating the potential contribution of mitochondrial
function to the etiology of type 2 diabetes. Numerous in vitro, in situ, and in vivo …

The pathogenic trypanosomatids Trypanosoma brucei, Trypanosoma cruzi, and Leishmania
spp. are the causative agents of African trypanosomiasis, Chagas disease, and …

Protocols for high-resolution respirometry (HRR) of intact cells, permeabilized cells, and
permeabilized muscle fibers offer sensitive diagnostic tests of integrated mitochondrial …

Abstract Analysis of mitochondrial function is central to the study of intracellular energy
metabolism, mechanisms of cell death and pathophysiology of a variety of human diseases …

Reactive oxygen species are a by-product of mitochondrial oxidative phosphorylation,
derived from a small quantity of superoxide radicals generated during electron transport. We …

The human kinome comprises 538 kinases playing essential functions by catalyzing protein
phosphorylation. Annotation of subcellular distribution of the kinome greatly facilitates …

Identifying metabolic steps that are specifically required for the survival of cancer cells but
are dispensable in normal cells remains a challenge. Here we report a therapeutic …

In this chapter we describe in details the permeabilized cell and skinned fiber techniques
and their applications for studies of mitochondrial function in vivo. The experience of more …

The use of digitonin to permeabilize the plasma membrane of Trypanosoma cruzi allowed
the identification of a non-mitochondrial nigericin-or bafilomycin A1-sensitive Ca (2+)-uptake …

Purpose. Dequalinium, a drug known for over 30 years, is a dicationic amphiphile
compound resembling bolaform electrolytes. The purpose of our work was to determine the …