Adoptive transfer of tumor-infiltrating lymphocytes (TILs) and genetically engineered T
lymphocytes expressing chimeric antigen receptors (CARs) or conventional alpha/beta T …

Mammalian cell cultures are increasingly used for the production of complex
biopharmaceuticals including viral vectors and vaccines. HEK293 is the predominant cell …

Lentiviral vectors (LVs) are promising tools for gene therapy. However, scaling up the
production methods of LVs in order to produce high-quality vectors for clinical purposes has …

Lentivirus can be engineered to be a highly potent vector for gene therapy applications.
However, generation of clinical grade vectors in enough quantities for therapeutic use is still …

Large-scale vector manufacturing for phase III and beyond has proven to be challenging.
Upscaling the process with suspension cells is increasingly feasible, but many viral …

The use of lentiviral vectors (LV) in gene therapy has been growing in recent years. To meet
the increasing clinical demand, LV production platforms will benefit from improved …

MicroRNAs (miRNAs) have emerged as promising targets for engineering of CHO cell
factories to enhance recombinant protein productivity. Manipulation of miRNA levels in CHO …

Gammaretroviral and lentiviral vectors (γ‐RV and LV) are among the most used vectors in
gene therapy. Currently, human embryonic kidney (HEK) 293 cells, the manufacture platform …

Gamma-retroviral vectors, commonly designated retroviral vectors, were the first viral vector
employed in Gene Therapy clinical trials in 1990 and are still one of the most used. More …

Adenoviruses are human pathogens increasingly used as gene therapy and vaccination
vectors. However, their impact on cell metabolism is poorly characterized. We performed …