Onsite detection of plant viruses using isothermal amplification assays

AI Bhat, R Aman, M Mahfouz - Plant biotechnology journal, 2022 - Wiley Online Library
Plant diseases caused by viruses limit crop production and quality, resulting in significant
losses. However, options for managing viruses are limited; for example, as systemic obligate …

Food safety in Post-COVID-19 pandemic: challenges and countermeasures

W Zhang, H He, L Zhu, G Liu, L Wu - Biosensors, 2021 - mdpi.com
Understanding food safety hazard risks is essential to avoid potential negative heath
impacts in the food supply chain in a post-COVID-19 pandemic era. Development of …

Development of reverse transcription recombinase polymerase amplification (RT-RPA): a methodology for quick diagnosis of potato leafroll viral disease in potato

R Kumar, P Kaundal, RK Tiwari, MK Lal… - International Journal of …, 2023 - mdpi.com
Potatoes are developed vegetatively from tubers, and therefore potato virus transmission is
always a possibility. The potato leafroll virus (PLRV) is a highly devastating virus of the …

Establishment of a one-step reverse transcription recombinase polymerase amplification assay for the detection of potato virus S

R Kumar, P Kaundal, RK Tiwari, S Siddappa… - Journal of Virological …, 2022 - Elsevier
Potato virus S (PVS) is a noteworthy threat to the propagation of healthy seed potatoes.
Accurate and speedy detection is critical for effective PVS management. In the present study …

Rapid and sensitive detection of potato virus X by one-step reverse transcription-recombinase polymerase amplification method in potato leaves and dormant tubers

R Kumar, P Kaundal, RK Tiwari, S Siddappa… - Molecular and cellular …, 2021 - Elsevier
Potato virus X (PVX), is a serious threat to global potato production. A simple and rapid
detection method is imperative for PVX diagnosis and early management. In this study, an …

[HTML][HTML] A rapid, low-cost deep learning system to classify strawberry disease based on cloud service

Y Guo-Feng, Y Yong, HE Zi-Kang, Z Xin-Yu… - Journal of integrative …, 2022 - Elsevier
Accurate and timely classification of diseases during strawberry planting can help growers
deal with them in timely manner, thereby reducing losses. However, the classification of …

Various techniques for molecular and rapid detection of infectious and epidemic diseases

MH Shahrajabian, W Sun - Letters in Organic Chemistry, 2023 - ingentaconnect.com
Polymerase chain reaction is an approach to make numerous copies of specific DNA. PCR
has been applied for the investigation of infectious sicknesses caused by viral, protozoan …

Development of a multiplex RT‐RPA assay for simultaneous detection of three viruses in cucurbits

AAK Jailani, ML Paret - Molecular Plant Pathology, 2023 - Wiley Online Library
Begomoviruses and criniviruses, vectored by whiteflies (Bemisia tabaci), are important
threats to crops worldwide. In recent years, the spread of cucurbit leaf crumple virus …

Development and application of crude sap-based recombinase polymerase amplification assay for the detection and occurrence of grapevine geminivirus A in Indian …

G Kishan, R Kumar, SK Sharma, N Srivastava… - Frontiers in Plant …, 2023 - frontiersin.org
Geminiviruses are known to infect several fields and horticultural crops around the globe.
Grapevine geminivirus A (GGVA) was reported in the United States in 2017, and since then …

Current status of recombinase polymerase amplification technologies for the detection of pathogenic microorganisms

S Zhang, M Duan, S Li, J Hou, T Qin, Z Teng… - … and Infectious Disease, 2023 - Elsevier
Rapid detection of pathogenic microorganisms is key to the epidemiologic identification,
prevention and control of disease in the field of public health. PCR-based pathogen …