Significance: Fluorescence lifetime imaging microscopy (FLIM) is a powerful technique to
distinguish the unique molecular environment of fluorophores. FLIM measures the time a …

Fluorescence lifetime imaging (FLIM) uses the fact that the fluorescence lifetime of a
fluorophore depends on its molecular environment but not on its concentration. Molecular …

Time-correlated single photon counting (TCSPC) is based on the detection of single photons
of a periodic light signal, measurement of the detection time of the photons, and the build-up …

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the
environment and interaction of fluorescent probes. It can report on photophysical events that …

We present a time‐correlated single photon counting (TCPSC) technique that allows time‐
resolved multi‐wavelength imaging in conjunction with a laser scanning microscope and a …

In fluorescence microscopy, the fluorescence emission can be characterised not only by
intensity and position, but also by lifetime, polarization and wavelength. Fluorescence …

We present applications of polar plots for analyzing fluorescence lifetime data acquired in
the frequency domain. This graphical, analytical method is especially useful for rapid FLIM …

Fluorescence lifetime imaging microscopy (FLIM) is increasingly used in biomedicine,
material science, chemistry, and other related research fields, because of its advantages of …

In the last decade or so, it has been realised that membranes do not just have a lipid-bilayer
structure in which proteins are embedded or with which they associate. Structures are …

Intensity-and amplitude-weighted average lifetimes, denoted as τ I and τ A hereafter, are
useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence …