Fluorescent organic small molecule probes for bioimaging and detection applications

Y Yang, F Gao, Y Wang, H Li, J Zhang, Z Sun, Y Jiang - Molecules, 2022 - mdpi.com
The activity levels of key substances (metal ions, reactive oxygen species, reactive nitrogen,
biological small molecules, etc.) in organisms are closely related to intracellular redox …

Label-free metabolic and structural profiling of dynamic biological samples using multimodal optical microscopy with sensorless adaptive optics

RR Iyer, JE Sorrells, L Yang, EJ Chaney… - Scientific reports, 2022 - nature.com
Label-free optical microscopy has matured as a noninvasive tool for biological imaging; yet,
it is criticized for its lack of specificity, slow acquisition and processing times, and weak and …

Photophysical engineering of fluorescent proteins: Accomplishments and challenges of physical chemistry strategies

S Mukherjee, R Jimenez - The Journal of Physical Chemistry B, 2022 - ACS Publications
Fluorescent proteins (FPs) have become ubiquitous tools for biological research and
concomitantly they are intriguing molecules that are amenable to study with a wide range of …

Osimertinib and anti-HER3 combination therapy engages immune dependent tumor toxicity via STING activation in trans

JM Vicencio, R Evans, R Green, Z An, J Deng… - Cell Death & …, 2022 - nature.com
Over the past decade, immunotherapy delivered novel treatments for many cancer types.
However, lung cancer still leads cancer mortality, and non-small-cell lung carcinoma …

Bridging high resolution sub-cellular imaging with physiologically relevant engineered tissues

Y Kargar Gaz Kooh, N Huebsch - Frontiers in Mechanical …, 2024 - frontiersin.org
While high-resolution microscopic techniques are crucial for studying cellular structures in
cell biology, obtaining such images from thick 3D engineered tissues remains challenging …

3D active stabilization for single-molecule imaging

S Coelho, J Baek, J Walsh, JJ Gooding, K Gaus - Nature Protocols, 2021 - nature.com
A key part of any super-resolution technique involves accurately correcting for mechanical
motion of the sample and setup during acquisition. If left uncorrected, drift degrades the …

The use of fluorescence lifetime imaging (FLIM) for in situ microbial detection in complex mineral substrates

Y Chmykh, JL Nadeau - Journal of Microscopy, 2024 - Wiley Online Library
The utility of fluorescence lifetime imaging microscopy (FLIM) for identifying bacteria in
complex mineral matrices was investigated. Baseline signals from unlabelled Bacillus …

Research on the transition dynamics and linear (nonlinear) optical properties of mCherry

Y Wang, G Li, J Dai, X Peng, Q Zhao - arXiv preprint arXiv:2304.11047, 2023 - arxiv.org
In this study, we explore the electron transition mechanism and optical properties of the
popular red fluorescent protein mCherry. By examining the charge transfer spectrum and …

Building a Total Internal Reflection Microscope (TIRF) with Active Stabilization (Feedback SMLM)

S Coelho, J Baek, JJ Gooding, K Gaus - Bio-protocol, 2021 - bio-protocol.org
The data quality of high-resolution imaging can be markedly improved with active
stabilization, which is based on feedback loops within the microscope that maintain the …

[PDF][PDF] Personal Contribution

A Eleftheriou, J Dernick, M Wyss, A Saab… - MR and Optical Imaging … - zora.uzh.ch
Two-photon excited fluorescence lifetime imaging (2P-FLIM) combined with genetically
encoded sensors (GESs) can greatly improve our understanding of metabolic processes in …