Flow cytometry based-FRET: basics, novel developments and future perspectives

JW Lim, M Petersen, M Bunz, C Simon… - Cellular and Molecular …, 2022 - Springer
Förster resonance energy transfer (FRET) is a widespread technology used to analyze and
quantify protein interactions in multiple settings. While FRET is traditionally measured by …

Factors influencing the choice of monoclonal antibodies for antibody–drug conjugates

MM Hasan, M Laws, P Jin, KM Rahman - Drug Discovery Today, 2022 - Elsevier
In antibody–drug conjugates (ADCs), monoclonal antibodies (mAbs) act as carriers for a
cytotoxic payload providing the therapy with targeted action against cells expressing a target …

SNAP-tagging live cells via chelation-assisted copper-catalyzed azide–alkyne cycloaddition

DJ Stone, M Macias-Contreras, SM Crist… - Organic & …, 2023 - pubs.rsc.org
SNAP-tag is a single-turnover enzyme that has become a powerful tool, hence a popular
choice, of targeted cellular protein labeling. Three SNAP-tag substrates that carry the copper …

Computerized fluorescence microscopy of microbial cells

EO Puchkov - World Journal of Microbiology and Biotechnology, 2021 - Springer
The upgrading of fluorescence microscopy by the introduction of computer technologies has
led to the creation of a new methodology, computerized fluorescence microscopy (CFM) …