Power to the protein: enhancing and combining activities using the Spy toolbox

AH Keeble, M Howarth - Chemical Science, 2020 - pubs.rsc.org
Proteins span an extraordinary range of shapes, sizes and functionalities. Therefore generic
approaches are needed to overcome this diversity and stream-line protein analysis or …

Structure and dynamics of interfacial peptides and proteins from vibrational sum-frequency generation spectroscopy

S Hosseinpour, SJ Roeters, M Bonn, W Peukert… - Chemical …, 2020 - ACS Publications
Proteins at interfaces play important roles in cell biology, immunology, bioengineering, and
biomimetic material design. Many biological processes are based on interfacial protein …

Cucurbit[n]uril-Based Microcapsules Self-Assembled within Microfluidic Droplets: A Versatile Approach for Supramolecular Architectures and Materials

J Liu, Y Lan, Z Yu, CSY Tan, RM Parker… - Accounts of Chemical …, 2017 - ACS Publications
Conspectus Microencapsulation is a fundamental concept behind a wide range of daily
applications ranging from paints, adhesives, and pesticides to targeted drug delivery …

Electrically induced bacterial membrane-potential dynamics correspond to cellular proliferation capacity

JP Stratford, CLA Edwards… - Proceedings of the …, 2019 - National Acad Sciences
Membrane-potential dynamics mediate bacterial electrical signaling at both intra-and
intercellular levels. Membrane potential is also central to cellular proliferation. It is unclear …

The past, present and future of protein-based materials

NC Abascal, L Regan - Royal Society Open Biology, 2018 - royalsocietypublishing.org
Protein-based materials are finding new uses and applications after millennia of impacting
the daily life of humans. Some of the earliest uses of protein-based materials are still evident …

Protein‐Based Encapsulation Strategies: Toward Micro‐and Nanoscale Carriers with Increased Functionality

R Ramos, J Bernard, F Ganachaud, A Miserez - Small Science, 2022 - Wiley Online Library
Proteins and peptides are attractive chemical building blocks to encapsulate and protect
active substances thanks to their biocompatibility, biodegradability, low immunogenicity, and …

[HTML][HTML] Nanoteamwork: covalent protein assembly beyond duets towards protein ensembles and orchestras

A Banerjee, M Howarth - Current opinion in biotechnology, 2018 - Elsevier
Highlights•Protein function can be extended by covalently ligating protein
modules.•Improved tools (eg sortase, intein, SpyTag, omniligase) extend ligation …

Extracellular Self-Assembly of Functional and Tunable Protein Conjugates from Bacillus subtilis

C Gilbert, M Howarth, CR Harwood… - ACS synthetic biology, 2017 - ACS Publications
The ability to stably and specifically conjugate recombinant proteins to one another is a
powerful approach for engineering multifunctional enzymes, protein therapeutics, and novel …

Bacillus subtilis Matrix Protein TasA is Interfacially Active, but BslA Dominates Interfacial Film Properties

RJ Morris, NC Bamford, KM Bromley, E Erskine… - Langmuir, 2024 - ACS Publications
Microbial growth often occurs within multicellular communities called biofilms, where cells
are enveloped by a protective extracellular matrix. Bacillus subtilis serves as a model …

Microfluidic assembly of food-grade delivery systems: Toward functional delivery structure design

Y Feng, Y Lee - Trends in Food Science & Technology, 2019 - Elsevier
Background Microfluidic process is an emerging technique that can be applied to fabricate
functional delivery systems with a precise control. Assembling food-grade delivery systems …