Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics

L Liu, X Dong, Y Tu, G Miao, Z Zhang, L Zhang… - Microfluidics and …, 2021 - Springer
Single-cell nucleic acid analysis aims at discovering the genetic differences between
individual cells which is well known as the cellular heterogeneity. This technology facilitates …

Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors

EC Wheeler, AQ Vu, JM Einstein, M DiSalvo… - Nature …, 2020 - nature.com
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive,
but restricted to standard readouts, including survival, proliferation and sortable markers …

A technology of a different sort: microraft arrays

B Cortés-Llanos, Y Wang, CE Sims, NL Allbritton - Lab on a Chip, 2021 - pubs.rsc.org
A common procedure performed throughout biomedical research is the selection and
isolation of biological entities such as organelles, cells and organoids from a mixed …

Optical manipulation of single magnetic beads in a microwell array on a digital microfluidic chip

D Decrop, T Brans, P Gijsenbergh, J Lu… - Analytical …, 2016 - ACS Publications
The detection of single molecules in magnetic microbead microwell array formats
revolutionized the development of digital bioassays. However, retrieval of individual …

Selective single cell isolation for genomics using microraft arrays

JD Welch, LA Williams, M DiSalvo… - Nucleic acids …, 2016 - academic.oup.com
Genomic methods are used increasingly to interrogate the individual cells that compose
specific tissues. However, current methods for single cell isolation struggle to phenotypically …

Unveiling the Potential of Single‐Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives

M Pires‐Santos, S Nadine, JF Mano - Small Science, 2024 - Wiley Online Library
The encapsulation of single cells has emerged as a promising field in recent years, owing to
its potential applications in cell‐based therapeutics, bioprinting, in vitro cell culture, high …

Microraft arrays for serial‐killer CD19 chimeric antigen receptor T cells and single cell isolation

CA LaBelle, RJ Zhang, SA Hunsucker… - Cytometry Part …, 2023 - Wiley Online Library
Chimeric antigen receptor T (CAR‐T) cell immunotherapies have seen success in treating
hematological malignancies in recent years; however, the results can be highly variable …

Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9

PJ Attayek, JP Waugh, SA Hunsucker… - Biosensors and …, 2017 - Elsevier
Microraft arrays have been used to screen and then isolate adherent and non-adherent cells
with very high efficiency and excellent viability; however, manual screening and isolation …

Characterization of tensioned PDMS membranes for imaging cytometry on microraft arrays

M DiSalvo, DM Harris, S Kantesaria, AN Peña… - Analytical …, 2018 - ACS Publications
Polydimethylsiloxane (PDMS) membranes can act as sensing elements, barriers, and
substrates, yet the low rigidity of the elastomeric membranes can limit their practical use in …

Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system

PJ Attayek, SA Hunsucker, CE Sims… - Integrative …, 2016 - academic.oup.com
The simultaneous measurement of T cell function with recovery of individual T cells would
greatly facilitate characterizing antigen-specific responses both in vivo and in model …