Methods and platforms for analysis of nucleic acids from single-cell based on microfluidics
L Liu, X Dong, Y Tu, G Miao, Z Zhang, L Zhang… - Microfluidics and …, 2021 - Springer
Single-cell nucleic acid analysis aims at discovering the genetic differences between
individual cells which is well known as the cellular heterogeneity. This technology facilitates …
individual cells which is well known as the cellular heterogeneity. This technology facilitates …
Pooled CRISPR screens with imaging on microraft arrays reveals stress granule-regulatory factors
EC Wheeler, AQ Vu, JM Einstein, M DiSalvo… - Nature …, 2020 - nature.com
Genetic screens using pooled CRISPR-based approaches are scalable and inexpensive,
but restricted to standard readouts, including survival, proliferation and sortable markers …
but restricted to standard readouts, including survival, proliferation and sortable markers …
A technology of a different sort: microraft arrays
B Cortés-Llanos, Y Wang, CE Sims, NL Allbritton - Lab on a Chip, 2021 - pubs.rsc.org
A common procedure performed throughout biomedical research is the selection and
isolation of biological entities such as organelles, cells and organoids from a mixed …
isolation of biological entities such as organelles, cells and organoids from a mixed …
Optical manipulation of single magnetic beads in a microwell array on a digital microfluidic chip
D Decrop, T Brans, P Gijsenbergh, J Lu… - Analytical …, 2016 - ACS Publications
The detection of single molecules in magnetic microbead microwell array formats
revolutionized the development of digital bioassays. However, retrieval of individual …
revolutionized the development of digital bioassays. However, retrieval of individual …
Selective single cell isolation for genomics using microraft arrays
Genomic methods are used increasingly to interrogate the individual cells that compose
specific tissues. However, current methods for single cell isolation struggle to phenotypically …
specific tissues. However, current methods for single cell isolation struggle to phenotypically …
Unveiling the Potential of Single‐Cell Encapsulation in Biomedical Applications: Current Advances and Future Perspectives
The encapsulation of single cells has emerged as a promising field in recent years, owing to
its potential applications in cell‐based therapeutics, bioprinting, in vitro cell culture, high …
its potential applications in cell‐based therapeutics, bioprinting, in vitro cell culture, high …
Microraft arrays for serial‐killer CD19 chimeric antigen receptor T cells and single cell isolation
CA LaBelle, RJ Zhang, SA Hunsucker… - Cytometry Part …, 2023 - Wiley Online Library
Chimeric antigen receptor T (CAR‐T) cell immunotherapies have seen success in treating
hematological malignancies in recent years; however, the results can be highly variable …
hematological malignancies in recent years; however, the results can be highly variable …
Automated microraft platform to identify and collect non-adherent cells successfully gene-edited with CRISPR-Cas9
PJ Attayek, JP Waugh, SA Hunsucker… - Biosensors and …, 2017 - Elsevier
Microraft arrays have been used to screen and then isolate adherent and non-adherent cells
with very high efficiency and excellent viability; however, manual screening and isolation …
with very high efficiency and excellent viability; however, manual screening and isolation …
Characterization of tensioned PDMS membranes for imaging cytometry on microraft arrays
Polydimethylsiloxane (PDMS) membranes can act as sensing elements, barriers, and
substrates, yet the low rigidity of the elastomeric membranes can limit their practical use in …
substrates, yet the low rigidity of the elastomeric membranes can limit their practical use in …
Identification and isolation of antigen-specific cytotoxic T lymphocytes with an automated microraft sorting system
PJ Attayek, SA Hunsucker, CE Sims… - Integrative …, 2016 - academic.oup.com
The simultaneous measurement of T cell function with recovery of individual T cells would
greatly facilitate characterizing antigen-specific responses both in vivo and in model …
greatly facilitate characterizing antigen-specific responses both in vivo and in model …