Protein‐protein interaction detection: methods and analysis

VS Rao, K Srinivas, GN Sujini… - International journal of …, 2014 - Wiley Online Library
Protein‐protein interaction plays key role in predicting the protein function of target protein
and drug ability of molecules. The majority of genes and proteins realize resulting …

Affinity‐purification coupled to mass spectrometry: Basic principles and strategies

WH Dunham, M Mullin, AC Gingras - Proteomics, 2012 - Wiley Online Library
Identifying the interactions established by a protein of interest can be a critical step in
understanding its function. This is especially true when an unknown protein of interest is …

Reducing stomatal density in barley improves drought tolerance without impacting on yield

J Hughes, C Hepworth, C Dutton, JA Dunn… - Plant …, 2017 - academic.oup.com
The epidermal patterning factor (EPF) family of secreted signaling peptides regulate the
frequency of stomatal development in model dicot and basal land plant species. Here, we …

Reduced stomatal density in bread wheat leads to increased water-use efficiency

J Dunn, L Hunt, M Afsharinafar… - Journal of …, 2019 - academic.oup.com
Wheat is a staple crop, frequently cultivated in water-restricted environments. Improving crop
water-use efficiency would be desirable if grain yield can be maintained. We investigated …

Gateway‐compatible vectors for plant functional genomics and proteomics

KW Earley, JR Haag, O Pontes, K Opper… - The Plant …, 2006 - Wiley Online Library
Gateway cloning technology facilitates high‐throughput cloning of target sequences by
making use of the bacteriophage lambda site‐specific recombination system. Target …

FKF1 conveys timing information for CONSTANS stabilization in photoperiodic flowering

YH Song, RW Smith, BJ To, AJ Millar, T Imaizumi - Science, 2012 - science.org
Plants use day-length information to coordinate flowering time with the appropriate season
to maximize reproduction. In Arabidopsis, the long day–specific expression of CONSTANS …

MYC2 Differentially Modulates Diverse Jasmonate-Dependent Functions in Arabidopsis

B Dombrecht, GP Xue, SJ Sprague… - The Plant …, 2007 - academic.oup.com
The Arabidopsis thaliana basic helix-loop-helix Leu zipper transcription factor (TF)
MYC2/JIN1 differentially regulates jasmonate (JA)-responsive pathogen defense (eg, PDF1 …

VelB/VeA/LaeA complex coordinates light signal with fungal development and secondary metabolism

O Bayram, S Krappmann, M Ni, JW Bok, K Helmstaedt… - Science, 2008 - science.org
Differentiation and secondary metabolism are correlated processes in fungi that respond to
light. In Aspergillus nidulans, light inhibits sexual reproduction as well as secondary …

Uncovering the protein translocon at the chloroplast inner envelope membrane

S Kikuchi, J Bédard, M Hirano, Y Hirabayashi, M Oishi… - Science, 2013 - science.org
Chloroplasts require protein translocons at the outer and inner envelope membranes,
termed TOC and TIC, respectively, to import thousands of cytoplasmically synthesized …

Structure–function analysis of the NB-ARC domain of plant disease resistance proteins

G Van Ooijen, G Mayr, MMA Kasiem… - Journal of …, 2008 - academic.oup.com
Resistance (R) proteins in plants are involved in pathogen recognition and subsequent
activation of innate immune responses. Most resistance proteins contain a central nucleotide …