[HTML][HTML] Genome engineering via gene editing technologies in microalgae
CRISPR-Cas has revolutionized genetic modification with its comparative simplicity and
accuracy, and it can be used even at the genomic level. Microalgae are excellent feedstocks …
accuracy, and it can be used even at the genomic level. Microalgae are excellent feedstocks …
Unconventional high-value products from microalgae: A review
Microalgae have gained significant importance in biotechnology development, providing
valuable goods and services in multiple applications. Although there is a rising market for …
valuable goods and services in multiple applications. Although there is a rising market for …
Challenges and advances towards the rational design of microalgal synthetic promoters in Chlamydomonas reinhardtii
Microalgae hold enormous potential to provide a safe and sustainable source of high-value
compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly …
compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly …
Gene delivery technologies with applications in microalgal genetic engineering
S Gutiérrez, KJ Lauersen - Biology, 2021 - mdpi.com
Simple Summary Microalgae and cyanobacteria are considered intriguing microbes for
sustainable biotechnological production of a wide array of high-value metabolites from …
sustainable biotechnological production of a wide array of high-value metabolites from …
Efficient gene replacement by CRISPR/Cas‐mediated homologous recombination in the model diatom Thalassiosira pseudonana
N Belshaw, I Grouneva, L Aram, A Gal… - New …, 2023 - Wiley Online Library
CRISPR/Cas enables targeted genome editing in many different plant and algal species
including the model diatom Thalassiosira pseudonana. However, efficient gene targeting by …
including the model diatom Thalassiosira pseudonana. However, efficient gene targeting by …
Molecular advancements establishing Chlamydomonas as a host for biotechnological exploitation
Chlamydomonas reinhardtii is emerging as a production platform for biotechnological
purposes thanks to recent achievements, which we briefly summarize in this review. Firstly …
purposes thanks to recent achievements, which we briefly summarize in this review. Firstly …
Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii
A Ferenczi, YP Chew, E Kroll, C von Koppenfels… - Nature …, 2021 - nature.com
Single-stranded oligodeoxynucleotides (ssODNs) are widely used as DNA repair templates
in CRISPR/Cas precision genome editing. However, the underlying mechanisms of single …
in CRISPR/Cas precision genome editing. However, the underlying mechanisms of single …
High Carotenoid Mutants of Chlorella vulgaris Show Enhanced Biomass Yield under High Irradiance
Microalgae represent a carbon-neutral source of bulk biomass, for extraction of high-value
compounds and production of renewable fuels. Due to their high metabolic activity and …
compounds and production of renewable fuels. Due to their high metabolic activity and …
Efficient methods for multiple types of precise gene‐editing in Chlamydomonas
H Chen, QL Yang, JX Xu, X Deng, YJ Zhang… - The Plant …, 2023 - Wiley Online Library
Precise gene‐editing using CRISPR/Cas9 technology remains a long‐standing challenge,
especially for genes with low expression and no selectable phenotypes in Chlamydomonas …
especially for genes with low expression and no selectable phenotypes in Chlamydomonas …
Harnessing the algal chloroplast for heterologous protein production
Photosynthetic microbes are gaining increasing attention as heterologous hosts for the light-
driven, low-cost production of high-value recombinant proteins. Recent advances in the …
driven, low-cost production of high-value recombinant proteins. Recent advances in the …