[HTML][HTML] Genome engineering via gene editing technologies in microalgae

B Jeong, J Jang, ES Jin - Bioresource Technology, 2023 - Elsevier
CRISPR-Cas has revolutionized genetic modification with its comparative simplicity and
accuracy, and it can be used even at the genomic level. Microalgae are excellent feedstocks …

Unconventional high-value products from microalgae: A review

S Abu-Ghosh, Z Dubinsky, V Verdelho, D Iluz - Bioresource Technology, 2021 - Elsevier
Microalgae have gained significant importance in biotechnology development, providing
valuable goods and services in multiple applications. Although there is a rising market for …

Challenges and advances towards the rational design of microalgal synthetic promoters in Chlamydomonas reinhardtii

A Milito, M Aschern, JL McQuillan… - Journal of Experimental …, 2023 - academic.oup.com
Microalgae hold enormous potential to provide a safe and sustainable source of high-value
compounds, acting as carbon-fixing biofactories that could help to mitigate rapidly …

Gene delivery technologies with applications in microalgal genetic engineering

S Gutiérrez, KJ Lauersen - Biology, 2021 - mdpi.com
Simple Summary Microalgae and cyanobacteria are considered intriguing microbes for
sustainable biotechnological production of a wide array of high-value metabolites from …

Efficient gene replacement by CRISPR/Cas‐mediated homologous recombination in the model diatom Thalassiosira pseudonana

N Belshaw, I Grouneva, L Aram, A Gal… - New …, 2023 - Wiley Online Library
CRISPR/Cas enables targeted genome editing in many different plant and algal species
including the model diatom Thalassiosira pseudonana. However, efficient gene targeting by …

Molecular advancements establishing Chlamydomonas as a host for biotechnological exploitation

M Schroda, C Remacle - Frontiers in Plant Science, 2022 - frontiersin.org
Chlamydomonas reinhardtii is emerging as a production platform for biotechnological
purposes thanks to recent achievements, which we briefly summarize in this review. Firstly …

Mechanistic and genetic basis of single-strand templated repair at Cas12a-induced DNA breaks in Chlamydomonas reinhardtii

A Ferenczi, YP Chew, E Kroll, C von Koppenfels… - Nature …, 2021 - nature.com
Single-stranded oligodeoxynucleotides (ssODNs) are widely used as DNA repair templates
in CRISPR/Cas precision genome editing. However, the underlying mechanisms of single …

High Carotenoid Mutants of Chlorella vulgaris Show Enhanced Biomass Yield under High Irradiance

Z Guardini, L Dall'Osto, S Barera, M Jaberi… - Plants, 2021 - mdpi.com
Microalgae represent a carbon-neutral source of bulk biomass, for extraction of high-value
compounds and production of renewable fuels. Due to their high metabolic activity and …

Efficient methods for multiple types of precise gene‐editing in Chlamydomonas

H Chen, QL Yang, JX Xu, X Deng, YJ Zhang… - The Plant …, 2023 - Wiley Online Library
Precise gene‐editing using CRISPR/Cas9 technology remains a long‐standing challenge,
especially for genes with low expression and no selectable phenotypes in Chlamydomonas …

Harnessing the algal chloroplast for heterologous protein production

EA Cutolo, G Mandalà, L Dall'Osto, R Bassi - Microorganisms, 2022 - mdpi.com
Photosynthetic microbes are gaining increasing attention as heterologous hosts for the light-
driven, low-cost production of high-value recombinant proteins. Recent advances in the …