Light sheet fluorescence microscopy
Light sheet fluorescence microscopy (LSFM) uses a thin sheet of light to excite only
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
fluorophores within the focal volume. Light sheet microscopes (LSMs) have a true optical …
Phototoxicity in live fluorescence microscopy, and how to avoid it
Phototoxicity frequently occurs during live fluorescence microscopy, and its consequences
are often underestimated. Damage to cellular macromolecules upon excitation light …
are often underestimated. Damage to cellular macromolecules upon excitation light …
Accurate and versatile 3D segmentation of plant tissues at cellular resolution
Quantitative analysis of plant and animal morphogenesis requires accurate segmentation of
individual cells in volumetric images of growing organs. In the last years, deep learning has …
individual cells in volumetric images of growing organs. In the last years, deep learning has …
Live imaging of root–bacteria interactions in a microfluidics setup
H Massalha, E Korenblum, S Malitsky… - Proceedings of the …, 2017 - National Acad Sciences
Plant roots play a dominant role in shaping the rhizosphere, the environment in which
interaction with diverse microorganisms occurs. Tracking the dynamics of root–microbe …
interaction with diverse microorganisms occurs. Tracking the dynamics of root–microbe …
Light-sheet fluorescence microscopy for quantitative biology
EHK Stelzer - Nature methods, 2015 - nature.com
In light sheet–based fluorescence microscopy (LSFM), optical sectioning in the excitation
process minimizes fluorophore bleaching and phototoxic effects. Because biological …
process minimizes fluorophore bleaching and phototoxic effects. Because biological …
An optogenetic gene expression system with rapid activation and deactivation kinetics
LB Motta-Mena, A Reade, MJ Mallory, S Glantz… - Nature chemical …, 2014 - nature.com
Optogenetic gene expression systems can control transcription with spatial and temporal
detail unequaled with traditional inducible promoter systems. However, current eukaryotic …
detail unequaled with traditional inducible promoter systems. However, current eukaryotic …
Light-sheet microscopy and its potential for understanding developmental processes
The ability to visualize and quantitatively measure dynamic biological processes in vivo and
at high spatiotemporal resolution is of fundamental importance to experimental …
at high spatiotemporal resolution is of fundamental importance to experimental …
Single cell optical imaging and spectroscopy
AS Stender, K Marchuk, C Liu, S Sander… - Chemical …, 2013 - ACS Publications
In his 1665 treatise, Micrographia, Robert Hooke described the many observations he had
made using a microscope, including compartment-like structures in cork samples that he …
made using a microscope, including compartment-like structures in cork samples that he …
Imaging fluorescence (cross-) correlation spectroscopy in live cells and organisms
Single-plane illumination (SPIM) or total internal reflection fluorescence (TIRF) microscopes
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …
can be combined with fast and single-molecule-sensitive cameras to allow spatially resolved …
The light-sheet microscopy revolution
JM Girkin, MT Carvalho - Journal of Optics, 2018 - iopscience.iop.org
This paper reviews the rapid advances that have been made in one form of optical biological
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …
imaging in the last decade, namely that of light sheet microscopy. Although the concept was …