[HTML][HTML] Huanglongbing pandemic: current challenges and emerging management strategies

D Ghosh, S Kokane, BK Savita, P Kumar, AK Sharma… - Plants, 2022 - mdpi.com
Huanglongbing (HLB, aka citrus greening), one of the most devastating diseases of citrus,
has wreaked havoc on the global citrus industry in recent decades. The culprit behind such …

A rapid and sensitive reverse transcription–loop-mediated isothermal amplification (RT-LAMP) assay for the detection of Indian Citrus Ringspot Virus

AD Kokane, SB Kokane, AJ Warghane… - Plant …, 2021 - Am Phytopath Society
Indian citrus ringspot virus (ICRSV) is a devastating pathogen that has a particularly
deleterious effect on the 'Kinnow mandarin', a commercial citrus crop cultivated in the …

[HTML][HTML] Development of a reverse transcription recombinase polymerase based isothermal amplification coupled with lateral flow immunochromatographic assay …

DK Ghosh, SB Kokane, S Gowda - Scientific reports, 2020 - nature.com
Tristeza is a highly destructive disease of citrus caused by the phloem-limited, flexuous
filamentous Citrus tristeza virus (CTV) in the genus Closterovirus and the family …

Detection and Molecular Characterization of 'Candidatus Liberibacter asiaticus' and Citrus Tristeza Virus Associated with Citrus Decline in Bhutan

DK Ghosh, AD Kokane, SB Kokane, J Tenzin… - …, 2021 - Am Phytopath Society
Citrus, mainly mandarin (Citrus reticulata Blanco), is an economically important fruit crop in
Bhutan. Despite having favorable agroclimatic conditions for citrus cultivation, the early …

Development of a SYBR Green-based RT-qPCR assay for the detection of Indian citrus ringspot virus

AD Kokane, K Lawrence, SB Kokane, MG Gubyad… - 3 Biotech, 2021 - Springer
The Indian citrus ringspot virus (ICRSV) that causes ringspot disease, especially to 'Kinnow
mandarin'hampers the sustainability of crop production. Presently, the disease is not …

Development of a real-time RT-PCR method for the detection of Citrus tristeza virus (CTV) and its implication in studying virus distribution in planta

SB Kokane, P Misra, AD Kokane, MG Gubyad… - 3 Biotech, 2021 - Springer
Tristeza is an economically important disease of the citrus caused by Citrus tristeza virus
(CTV) of genus Closterovirus and family Closteroviridae. The disease has caused …

In-silico characterization and RNA-binding protein based polyclonal antibodies production for detection of citrus tristeza virus

SB Kokane, AD Kokane, P Misra, AJ Warghane… - Molecular and cellular …, 2020 - Elsevier
Citrus tristeza virus (CTV) is the etiologic agent of the destructive Tristeza disease, a
massive impediment for the healthy citrus industry worldwide. Routine indexing of CTV is an …

[HTML][HTML] A comprehensive analysis of citrus tristeza variants of Bhutan and across the world

DK Ghosh, A Kokane, S Kokane, K Mukherjee… - Frontiers in …, 2022 - frontiersin.org
Mandarin orange is economically one of the most important fruit crops in Bhutan. However,
in recent years, orange productivity has dropped due to severe infection of citrus tristeza …

[HTML][HTML] Quick Plant Sample Preparation Methods Using a Micro-Homogenizer for the Detection of Multiple Citrus Pathogens

CW Liu, S Bodaghi, G Vidalakis, H Tsutsui - Chemosensors, 2024 - mdpi.com
Effective pathogen detection is essential for plant disease control. However, plant sample
preparation for downstream assays, such as quantitative polymerase chain reaction (qPCR) …

Development of multiplex PCR based diagnostics assay for simultaneous detection of major citrus pathogens

RD Bharsakale, BK Alex, MG Gubyad, SB Kokane… - Indian …, 2024 - Springer
Citrus, most economically significant fruit crop globally and represents India's third largest
fruit industry after banana and mango. Susceptibility of citrus spp. to more than 150 diseases …