Sperm oxidative stress during in vitro manipulation and its effects on sperm function and embryo development

R Gualtieri, G Kalthur, V Barbato, S Longobardi… - Antioxidants, 2021 - mdpi.com
Reactive oxygen species (ROS) generated at low levels during mitochondrial respiration
have key roles in several signaling pathways. Oxidative stress (OS) arises when the …

The effect of cryopreservation on the genome of gametes and embryos: principles of cryobiology and critical appraisal of the evidence

J Kopeika, A Thornhill, Y Khalaf - Human reproduction update, 2015 - academic.oup.com
BACKGROUND Cryopreservation has been extensively used in assisted reproductive
technology, agriculture and conservation programmes for endangered species. The …

In vitro comparison of egg yolk–based and soybean lecithin–based extenders for cryopreservation of ram semen

M Forouzanfar, M Sharafi, SM Hosseini… - Theriogenology, 2010 - Elsevier
Substitution of egg yolk with soybean lecithin may reduce hygienic risks in extenders.
Though a few studies have been performed on the effect of soybean lecithin in bull, to date …

Cryopreservation of the germplasm of animals used in biological and medical research: importance, impact, status, and future directions

P Mazur, SP Leibo, GE Seidel Jr - Biology of reproduction, 2008 - academic.oup.com
Molecular genetics and developmental biology have created thousands of new strains of
laboratory animals, including rodents, Drosophila, and zebrafish. This process will …

Human sperm vitrification: A scientific report

M Schulz, J Risopatrón, P Uribe, E Isachenko… - …, 2020 - Wiley Online Library
Background The sperm vitrification developed by this group is based on the ultrarapid
freezing of a vitrification solution composed of a non‐permeable cryoprotectant (saccharides …

DNA fragmentation index (DFI) as a measure of sperm quality and fertility in mice

MW Li, KCK Lloyd - Scientific reports, 2020 - nature.com
Although thousands of genetically modified mouse strains have been cryopreserved by
sperm freezing, the likelihood of cryorecovery success cannot be accurately predicted using …

Improved post-thaw quality of canine semen after treatment with exosomes from conditioned medium of adipose-derived mesenchymal stem cells

AY Qamar, X Fang, MJ Kim, J Cho - Animals, 2019 - mdpi.com
Simple Summary In this study, we have tried to exploit the potential role of exosomes-
derived from canine adipose-derived mesenchymal stem cells in the protection and repair of …

Inhibiting ice recrystallization and optimization of cell viability after cryopreservation

JL Chaytor, JM Tokarew, LK Wu, M Leclère… - …, 2012 - academic.oup.com
The ice recrystallization inhibition activity of various mono-and disaccharides has been
correlated with their ability to cryopreserve human cell lines at various concentrations. Cell …

[HTML][HTML] Role of male factor in early recurrent embryo loss: do antioxidants have any effect?

AM Gil-Villa, W Cardona-Maya, A Agarwal, R Sharma… - Fertility and sterility, 2009 - Elsevier
OBJECTIVE: To evaluate whether increasing antioxidant intake in men with high levels of
DNA damage or lipid peroxidation improves gestational results in couples with history of …

Conserving, distributing and managing genetically modified mouse lines by sperm cryopreservation

GC Ostermeier, MV Wiles, JS Farley, RA Taft - PloS one, 2008 - journals.plos.org
Background Sperm from C57BL/6 mice are difficult to cryopreserve and recover. Yet, the
majority of genetically modified (GM) lines are maintained on this genetic background …