Diverse classes of RNA, ranging from small to long non-coding RNAs, have emerged as key
regulators of gene expression, genome stability and defence against foreign genetic …

The character of a cell is defined by its constituent proteins, which are the result of specific
patterns of gene expression. Crucial determinants of gene expression patterns are DNA …

Double-stranded RNA-mediated interference (RNAi) is a simple and rapid method of
silencing gene expression in a range of organisms. The silencing of a gene is a …

Small interfering RNAs (siRNAs) are the mediators of mRNA degradation in the process of
RNA interference (RNAi). Here, we describe a human biochemical system that recapitulates …

RNA interference (RNAi) is a widespread silencing mechanism that acts at both the
posttranscriptional and transcriptional levels. Here, we describe the purification of an RNAi …

Mutations in the Arabidopsis ROS1 locus cause transcriptional silencing of a transgene and
a homologous endogenous gene. In the ros1 mutants, the promoter of the silenced loci is …

Proteins of the ARGONAUTE family are important in diverse posttranscriptional RNA-
mediated gene-silencing systems as well as in transcriptional gene silencing in Drosophila …

Eukaryotic DNA is organized into structurally distinct domains that regulate gene expression
and chromosome behavior. Epigenetically heritable domains of heterochromatin control the …

The function of plant genomes depends on chromatin marks such as the methylation of DNA
and the post-translational modification of histones. Techniques for studying model plants …

Proper DNA methylation patterning requires the complementary processes of de novo
methylation (the initial methylation of unmethylated DNA sequences) and maintenance …