As part of its remit to provide computational support to the cryo-EM community, the
Collaborative Computational Project for Electron cryo-Microscopy (CCP-EM) has produced …

This review summarizes the current state of methods and results achievable by cryo-electron
microscopy (cryo-EM) imaging for molecular, cell, and structural biologists who wish to …

This paper introduces ISOLDE, a new software package designed to provide an intuitive
environment for high-fidelity interactive remodelling/refinement of macromolecular models …

Cryogenic electron microscopy (cryo-EM) maps are now at the point where resolvability of
individual atoms can be achieved. However, resolvability is not necessarily uniform …

An algorithm for automatic map sharpening is presented that is based on optimization of the
detail and connectivity of the sharpened map. The detail in the map is reflected in the …

Atomic models based on high-resolution density maps are the ultimate result of the cryo-EM
structure determination process. Here, we introduce a general procedure for local …

This paper describes outcomes of the 2019 Cryo-EM Model Challenge. The goals were to
(1) assess the quality of models that can be produced from cryogenic electron microscopy …

Cryo-electron microscopy (cryo-EM) has become a leading approach for protein structure
determination, but it remains challenging to accurately model atomic structures with cryo-EM …

Refolding aggregated proteins is essential in combating cellular proteotoxic stress. Together
with Hsp70, Hsp100 chaperones, including Escherichia coli ClpB, form a powerful …

Cryo-EM experiments produce images of macromolecular assemblies that are combined to
produce three-dimensional density maps. Typically, atomic models of the constituent …