Animal cell-based expression platforms enable the production of complex biomolecules
such as recombinant proteins and viral vectors. Although most biotherapeutics are produced …

Genetically engineered T cells have sparked interest in advanced cancer treatment,
reaching a milestone in 2017 with two FDA-approvals for CD19-directed chimeric antigen …

CAR‐T therapy is a particularly effective treatment for some types of cancer that uses
retroviruses to deliver the gene for a chimeric antigen receptor (CAR) to a patient's T cells ex …

Retroviral vectors derived from murine leukemia virus (MLV) are used in somatic gene
therapy applications eg for genetic modification of hematopoietic stem cells. Recently, we …

Mammalian cell cultures are increasingly used for the production of complex
biopharmaceuticals including viral vectors and vaccines. HEK293 is the predominant cell …

Highlights•Stable packaging cell lines have not yet met the growing demand for viral
vectors.•Viral plasmid systems have been developed to improve viral titers and …

Lentiviral vectors (LVs) are excellent tools for gene transfer into mammalian cells. It is
noteworthy that the first gene therapy treatment using LVs was approved for …

Retroviral vectors derived from murine leukemia virus (MLV) are amongst the most
frequently utilized vectors in gene therapy approaches such as the genetic modification of …

Transfection of surface adherent cells remain as a standard methodology for lentiviral
production for early phase clinical studies and research purposes. Production today is …

In this study, a chemically defined, animal component-free media was developed to promote
Vero growth in suspension. Key media compounds were screened using Plackett–Burman …