Voltage imaging to understand connections and functions of neuronal circuits

SD Antic, RM Empson… - Journal of …, 2016 - journals.physiology.org
Understanding of the cellular mechanisms underlying brain functions such as cognition and
emotions requires monitoring of membrane voltage at the cellular, circuit, and system levels …

Primer to voltage imaging with ANNINE dyes and two-photon microscopy

B Kuhn, CJ Roome - Frontiers in Cellular Neuroscience, 2019 - frontiersin.org
ANNINE-6 and ANNINE-6plus are voltage-sensitive dyes that when combined with two-
photon microscopy are ideal for recording of neuronal voltages in vivo, in both bulk loaded …

Simultaneous dendritic voltage and calcium imaging and somatic recording from Purkinje neurons in awake mice

CJ Roome, B Kuhn - Nature communications, 2018 - nature.com
Spatiotemporal maps of dendritic signalling and their relationship with somatic output is
fundamental to neuronal information processing, yet remain unexplored in awake animals …

Compartmentalized calcium signaling in cilia regulates intraflagellar transport

P Collingridge, C Brownlee, GL Wheeler - Current Biology, 2013 - cell.com
Intraflagellar transport (IFT) underpins many of the important cellular roles of cilia and
flagella in signaling and motility [1–4]. The microtubule motors kinesin-2 and cytoplasmic …

Membrane potential measurements of isolated neurons using a voltage-sensitive dye

R Fairless, A Beck, M Kravchenko, SK Williams… - PloS one, 2013 - journals.plos.org
The ability to monitor changes in membrane potential is a useful tool for studying neuronal
function, but there are only limited options available at present. Here, we have investigated …

Voltage imaging with ANNINE dyes and two-photon microscopy of Purkinje dendrites in awake mice

CJ Roome, B Kuhn - Neuroscience Research, 2020 - Elsevier
Voltage imaging is the next generation of functional imaging in neuroscience. It promises to
resolve neuronal activity 10 to 100-times faster than calcium imaging and to report not only …

Voltage imaging with ANNINE dyes and two-photon microscopy

CJ Roome, B Kuhn - Multiphoton Microscopy, 2019 - Springer
Voltage imaging is a tried and tested tool for revealing changes in neuronal membrane
voltage at high temporal and spatial resolution in vitro and in vivo. However, single-photon …

Imaging different cell populations in the mouse olfactory bulb using the genetically encoded voltage indicator ArcLight

LM Leong, DA Storace - Neurophotonics, 2024 - spiedigitallibrary.org
Genetically encoded voltage indicators (GEVIs) are protein-based optical sensors that allow
for measurements from genetically defined populations of neurons. Although in vivo imaging …

Optical detection of neuron connectivity by random access two-photon microscopy

N Shafeghat, M Heidarinejad, N Murata… - Journal of Neuroscience …, 2016 - Elsevier
Background Knowledge about the distribution, strength, and direction of synaptic
connections within neuronal networks are crucial for understanding brain function …

Advanced real-time recordings of neuronal activity with tailored patch pipettes, diamond multi-electrode arrays and electrochromic voltage-sensitive dyes

B Kuhn, F Picollo, V Carabelli, G Rispoli - Pflügers Archiv-European …, 2021 - Springer
To understand the working principles of the nervous system is key to figure out its electrical
activity and how this activity spreads along the neuronal network. It is therefore crucial to …