Emerging tumor-on-chips with electrochemical biosensors

L Lei, B Ma, C Xu, H Liu - TrAC Trends in Analytical Chemistry, 2022 - Elsevier
Cancer, one of the most dangerous diseases with a high mortality rate, has attracted much
attention and interest. Advances in tissue engineering and microfluidics have led to the …

Recent methods of droplet microfluidics and their applications in spheroids and organoids

Y Wang, M Liu, Y Zhang, H Liu, L Han - Lab on a Chip, 2023 - pubs.rsc.org
Droplet microfluidic techniques have long been known as a high-throughput approach for
cell manipulation. The capacity to compartmentalize cells into picolitre droplets in …

Spheroid engineering in microfluidic devices

A Tevlek, S Kecili, OS Ozcelik, H Kulah, HC Tekin - ACS omega, 2023 - ACS Publications
Two-dimensional (2D) cell culture techniques are commonly employed to investigate
biophysical and biochemical cellular responses. However, these culture methods, having …

Multidimensional controllable fabrication of tumor spheroids based on a microfluidic device

Y Hou, Y Zheng, X Zheng, Y Sun, X Yi, Z Wu, JM Lin - Lab on a Chip, 2023 - pubs.rsc.org
Multicellular tumor spheroids (MCTSs) are in vitro solid tumor models with physiological
relevance. To achieve robust process control, a MCTS fabrication method that combines cell …

Advances of three-dimensional (3D) culture systems for in vitro spermatogenesis

M Salem, F Khadivi, P Javanbakht… - Stem Cell Research & …, 2023 - Springer
The loss of germ cells and spermatogenic failure in non-obstructive azoospermia are
believed to be the main causes of male infertility. Laboratory studies have used in vitro …

Thermo-Induced Coalescence of Dual Cores in Double Emulsions for Single-Cell RT-PCR

F Qu, L Zhao, L Li, S Zhao, M Yang, J Yu… - Analytical …, 2022 - ACS Publications
Single-cell reverse-transcription polymerase chain reaction (RT-PCR) has shown significant
promise for transcriptional profiling of heterogeneous cells. However, currently developed …

Reflow-molded deep concave microwell arrays for robust and large-scale production of embryoid bodies

X Han, Q Zhang, H He, Q Zhao, G Li - Lab on a Chip, 2023 - pubs.rsc.org
Embryonic stem cell (ESC)-derived aggregates, called embryoid bodies (EBs), are powerful
in vitro models used to study human development and disease. However, the cost-effective …

3D cell cultures toward quantitative high-throughput drug screening

Y Wang, H Jeon - Trends in Pharmacological Sciences, 2022 - cell.com
3D cell cultures are being utilized for drug discovery and development. However, there are
still challenges to implementing them generally in quantitative high-throughput screening …

Flow cytometric printing of double emulsions into open droplet arrays

P Zhang, L Xu, H Chen, AR Abate - Lab on a Chip, 2023 - pubs.rsc.org
Delivery of double emulsions in air is crucial for their applications in mass spectrometry,
bioanalytics, and material synthesis. However, while methods have been developed to …

Microfabrication methods for 3D spheroids formation and their application in biomedical engineering

S Ahn, D Kim, K Cho, WG Koh - Korean Journal of Chemical Engineering, 2023 - Springer
Three-dimensional cell culture systems offer greater understanding of the complex human
body structure than monolayer cell cultures. Spheroids, which are the most useful and …