In contrast to conventional therapies that require repeated dosing, gene therapy can treat
diseases by correcting defective genes after a single transfection and achieving cascade …

Multiplexed genetic perturbations are critical for testing functional interactions among coding
or non-coding genetic elements. Compared to double-stranded DNA cutting, repressive …

Background Somatic copy number alterations are a hallmark of cancer that offer unique
opportunities for therapeutic exploitation. Here, we focused on the identification of specific …

基因重复指基因组中一个基因通过多样化的分子机制从一个基因拷贝形成两个或多个重复拷贝
的过程, 是新基因起源的重要途径之一, 对真核生物基因组贡献了约为一半的基因 …

Interactions between multiple genes or cis-regulatory elements (CREs) underlie a wide
range of biological processes in both health and disease. High-throughput screens using …

Proteins operate within dense interconnected networks, where interactions are necessary
both for stabilising proteins and for enabling them to execute their molecular functions …

Cas12a is a gene-editing tool that simplifies multiplexed gene targeting through its RNase
activity, enabling maturation of individual crRNAs from a pre-crRNA-encoding RNA. Here …

Cell atlas projects have nominated recurrent transcriptional states as drivers of biological
processes and disease, but their origins, regulation, and properties remain unclear. To …

Buffering between genes is fundamental for robust cellular functions. While experimentally
testing all possible gene pairs is infeasible, gene buffering can be predicted genome-wide …

Background: Synthetic lethality offers a promising strategy for cancer treatment by targeting
genetic vulnerabilities unique to tumor cells, leading to selective tumor cell death. However …