Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins
J Arnau, C Lauritzen, GE Petersen… - Protein expression and …, 2006 - Elsevier
Affinity tags are highly efficient tools for protein purification. They allow the purification of
virtually any protein without any prior knowledge of its biochemical properties. The use of …
virtually any protein without any prior knowledge of its biochemical properties. The use of …
Plants as bioreactors: recent developments and emerging opportunities
In recent years, the use of plants as bioreactors has emerged as an exciting area of research
and significant advances have created new opportunities. The driving forces behind the …
and significant advances have created new opportunities. The driving forces behind the …
Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme
INTRODUCTION Discrimination of self from nonself is pivotal for cellular organisms, as it
allows the perception of pathogenic invaders that might otherwise multiply unchecked and …
allows the perception of pathogenic invaders that might otherwise multiply unchecked and …
Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to
detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 …
detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 …
Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagation
U Dubiella, H Seybold, G Durian… - Proceedings of the …, 2013 - National Acad Sciences
In animals and plants, pathogen recognition triggers the local activation of intracellular
signaling that is prerequisite for mounting systemic defenses in the whole organism. We …
signaling that is prerequisite for mounting systemic defenses in the whole organism. We …
A core function of EDS1 with PAD4 is to protect the salicylic acid defense sector in Arabidopsis immunity
H Cui, E Gobbato, B Kracher, J Qiu, J Bautor… - New …, 2017 - Wiley Online Library
Plant defenses induced by salicylic acid (SA) are vital for resistance against biotrophic
pathogens. In basal and receptor‐triggered immunity, SA accumulation is promoted by …
pathogens. In basal and receptor‐triggered immunity, SA accumulation is promoted by …
A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors
Plant nucleotide binding/leucine-rich repeat (NLR) immune receptors are activated by
pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain …
pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain …
Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 Function in Abscisic Acid-Mediated Signaling and H2O2 Homeostasis in Stomatal Guard …
JJ Zou, XD Li, D Ratnasekera, C Wang, WX Liu… - The Plant …, 2015 - academic.oup.com
Drought is a major threat to plant growth and crop productivity. Calcium-dependent protein
kinases (CDPKs, CPKs) are believed to play important roles in plant responses to drought …
kinases (CDPKs, CPKs) are believed to play important roles in plant responses to drought …
Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation
M Walter, C Chaban, K Schütze, O Batistic… - The Plant …, 2004 - Wiley Online Library
Dynamic networks of protein–protein interactions regulate numerous cellular processes and
determine the ability to respond appropriately to environmental stimuli. However, the …
determine the ability to respond appropriately to environmental stimuli. However, the …
The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants
In plants, host response to pathogenic microbes is driven both by microbial perception and
detection of modified-self. The Xanthomonas campestris effector protein AvrAC/XopAC …
detection of modified-self. The Xanthomonas campestris effector protein AvrAC/XopAC …