Current strategies for the use of affinity tags and tag removal for the purification of recombinant proteins

J Arnau, C Lauritzen, GE Petersen… - Protein expression and …, 2006 - Elsevier
Affinity tags are highly efficient tools for protein purification. They allow the purification of
virtually any protein without any prior knowledge of its biochemical properties. The use of …

Plants as bioreactors: recent developments and emerging opportunities

AK Sharma, MK Sharma - Biotechnology advances, 2009 - Elsevier
In recent years, the use of plants as bioreactors has emerged as an exciting area of research
and significant advances have created new opportunities. The driving forces behind the …

Direct pathogen-induced assembly of an NLR immune receptor complex to form a holoenzyme

S Ma, D Lapin, LI Liu, Y Sun, W Song, X Zhang… - Science, 2020 - science.org
INTRODUCTION Discrimination of self from nonself is pivotal for cellular organisms, as it
allows the perception of pathogenic invaders that might otherwise multiply unchecked and …

Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity

X Sun, D Lapin, JM Feehan, SC Stolze… - Nature …, 2021 - nature.com
Plants utilise intracellular nucleotide-binding, leucine-rich repeat (NLR) immune receptors to
detect pathogen effectors and activate local and systemic defence. NRG1 and ADR1 …

Calcium-dependent protein kinase/NADPH oxidase activation circuit is required for rapid defense signal propagation

U Dubiella, H Seybold, G Durian… - Proceedings of the …, 2013 - National Acad Sciences
In animals and plants, pathogen recognition triggers the local activation of intracellular
signaling that is prerequisite for mounting systemic defenses in the whole organism. We …

A core function of EDS1 with PAD4 is to protect the salicylic acid defense sector in Arabidopsis immunity

H Cui, E Gobbato, B Kracher, J Qiu, J Bautor… - New …, 2017 - Wiley Online Library
Plant defenses induced by salicylic acid (SA) are vital for resistance against biotrophic
pathogens. In basal and receptor‐triggered immunity, SA accumulation is promoted by …

A coevolved EDS1-SAG101-NRG1 module mediates cell death signaling by TIR-domain immune receptors

D Lapin, V Kovacova, X Sun, JA Dongus… - The Plant …, 2019 - academic.oup.com
Plant nucleotide binding/leucine-rich repeat (NLR) immune receptors are activated by
pathogen effectors to trigger host defenses and cell death. Toll-interleukin 1 receptor domain …

Arabidopsis CALCIUM-DEPENDENT PROTEIN KINASE8 and CATALASE3 Function in Abscisic Acid-Mediated Signaling and H2O2 Homeostasis in Stomatal Guard …

JJ Zou, XD Li, D Ratnasekera, C Wang, WX Liu… - The Plant …, 2015 - academic.oup.com
Drought is a major threat to plant growth and crop productivity. Calcium-dependent protein
kinases (CDPKs, CPKs) are believed to play important roles in plant responses to drought …

Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation

M Walter, C Chaban, K Schütze, O Batistic… - The Plant …, 2004 - Wiley Online Library
Dynamic networks of protein–protein interactions regulate numerous cellular processes and
determine the ability to respond appropriately to environmental stimuli. However, the …

The decoy substrate of a pathogen effector and a pseudokinase specify pathogen-induced modified-self recognition and immunity in plants

G Wang, B Roux, F Feng, E Guy, L Li, N Li, X Zhang… - Cell host & …, 2015 - cell.com
In plants, host response to pathogenic microbes is driven both by microbial perception and
detection of modified-self. The Xanthomonas campestris effector protein AvrAC/XopAC …