Nuclear pore complexes (NPCs) are multiprotein assemblies embedded within the nuclear
envelope and involved in the control of the bidirectional transport of proteins and …

Background Spatial transcriptomics are a set of new technologies that profile gene
expression on tissues with spatial localization information. With technological advances …

Accessing information on an underlying network driving a biological process often involves
interrupting the process and collecting snapshot data. When snapshot data are stochastic …

We describe a flow-cytometry-based protocol for intracellular mRNA measurements in
nonadherent mammalian cells using fluorescence in situ hybridization (FISH) probes. The …

Introduction In situ hybridization (ISH) plays an important role in the field of molecular
diagnostics, especially in an anatomical pathology laboratory. ISH is a technique that can …

In the last decade, multiple studies demonstrated that cells maintain a balance of mRNA
production and degradation, but the mechanisms by which cells implement this balance …

Gene duplication promotes adaptive evolution in two main ways: allowing one duplicate to
evolve a new function and splitting ancestral functions between the duplicates. The second …

The cellular response to genotoxic stress is mediated by a well-characterized network of
DNA surveillance pathways. The contribution of post-transcriptional gene regulatory …

After synthesis and transit through the nucleus, messenger RNAs (mRNAs) are exported to
the cytoplasm through the nuclear pore complex (NPC). At the NPC, messenger …

Single molecule fluorescence in situ hybridization (smFISH) is a powerful technique to study
gene expression in single cells due to its ability to detect and count individual RNA …