Advances in mass spectrometry to unravel the structure and function of protein condensates

C Sahin, A Leppert, M Landreh - Nature Protocols, 2023 - nature.com
Membrane-less organelles assemble through liquid–liquid phase separation (LLPS) of
partially disordered proteins into highly specialized microenvironments. Currently, it is …

Biomolecular condensates and disease pathogenesis

K Ruan, G Bai, Y Fang, D Li, T Li, X Liu, B Lu… - Science China Life …, 2024 - Springer
Biomolecular condensates or membraneless organelles (MLOs) formed by liquid-liquid
phase separation (LLPS) divide intracellular spaces into discrete compartments for specific …

HDX–MS finds that partial unfolding with sequential domain activation controls condensation of a cellular stress marker

R Chen, H Glauninger, DN Kahan… - Proceedings of the …, 2024 - National Acad Sciences
Eukaryotic cells form condensates to sense and adapt to their environment [SF Banani, HO
Lee, AA Hyman, MK Rosen, Nat. Rev. Mol. Cell Biol. 18, 285–298 (2017), H. Yoo, C …

Liquid–liquid phase separation primes spider silk proteins for fiber formation via a conditional sticker domain

A Leppert, G Chen, D Lama, C Sahin, V Railaite… - Nano Letters, 2023 - ACS Publications
Many protein condensates can convert to fibrillar aggregates, but the underlying
mechanisms are unclear. Liquid–liquid phase separation (LLPS) of spider silk proteins …

Ion Mobility mass spectrometry unveils global protein conformations in response to conditions that promote and reverse liquid–liquid phase separation

CG Robb, TP Dao, J Ujma, CA Castañeda… - Journal of the …, 2023 - ACS Publications
Liquid–liquid phase separation (LLPS) is a process by which biomacromolecules,
particularly proteins, condense into a dense phase that resembles liquid droplets …

Liquid-liquid phase separation in human diseases: Functions, mechanisms and treatments

T Yue, F Zhang, Y Wei, Z Wang - Nano Today, 2024 - Elsevier
Cells are separated into functional regions by non-membrane organelles and membrane-
enclosed organelles, ensuring that various cellular activities occur in a controlled manner in …

Liquid-liquid Phase Separation in Aging: Novel Insights in the Pathogenesis and Therapeutics

H Wang, J Tang, S Yan, C Li, Z Li, Z Xiong, Z Li… - Ageing Research …, 2024 - Elsevier
The intricate organization of distinct cellular compartments is paramount for the maintenance
of normal biological functions and the orchestration of complex biochemical reactions …

Emerging experimental methods to study the thermodynamics of biomolecular condensate formation

S Ray, AK Buell - The Journal of Chemical Physics, 2024 - pubs.aip.org
The formation of biomolecular condensates in vivo is increasingly recognized to underlie a
multitude of crucial cellular functions. Furthermore, the evolution of highly dynamic protein …

Phase separation in DNA double-strand break response

HL Liu, H Nan, WW Zhao, XB Wan, XJ Fan - Nucleus, 2024 - Taylor & Francis
ABSTRACT DNA double-strand break (DSB) is the most dangerous type of DNA damage,
which may lead to cell death or oncogenic mutations. Homologous recombination (HR) and …

Major structural protein in locust mandible capable of forming extraordinarily stiff materials via hierarchical self-assembly

H Qi, Y Teng, S Chen, F Yuan, L Chen, X Liang… - Matter, 2024 - cell.com
Locust mandibles have stiff cuticles to process rough food. Therefore, the mandible is a
reservoir of stiff structural proteins (SPs). Here, LmCPH-1, the most abundant SP in locust …