Recent advances in gene therapy: genetic bullets to the root of the problem

M Danaeifar - Clinical and Experimental Medicine, 2023 - Springer
Genetics and molecular genetic techniques have changed many perspectives and
paradigms in medicine. Using genetic methods, many diseases have been cured or …

Clostridioides difficile–phage relationship the RNA way

V Kreis, O Soutourina - Current Opinion in Microbiology, 2022 - Elsevier
Highlights•C. difficile (CD) is an emerging human enteropathogen.•Recent advances
suggest a key role of RNAs in CD and its interplay with phages.•CD prophages encode …

Structure and genome editing of type IB CRISPR-Cas

M Lu, C Yu, Y Zhang, W Ju, Z Ye, C Hua, J Mao… - Nature …, 2024 - nature.com
Abstract Type I CRISPR-Cas systems employ multi-subunit effector Cascade and helicase-
nuclease Cas3 to target and degrade foreign nucleic acids, representing the most abundant …

Identification of an anti-CRISPR protein that inhibits the CRISPR-Cas type IB system in Clostridioides difficile

P Muzyukina, A Shkaruta, NM Guzman, J Andreani… - Msphere, 2023 - Am Soc Microbiol
ABSTRACT CRISPR-Cas systems provide prokaryotic hosts with adaptive immunity against
mobile genetic elements. Many bacteriophages encode anti-CRISPR (Acr) proteins that …

Dual RNA-seq study of the dynamics of coding and non-coding RNA expression during Clostridioides difficile infection in a mouse model

V Kreis, C Toffano-Nioche, C Denève-Larrazet… - …, 2024 - journals.asm.org
Clostridioides difficile is the leading cause of healthcare-associated diarrhea in
industrialized countries. Many questions remain to be answered about the mechanisms …

[HTML][HTML] Research Progress on the Mechanism and Application of the Type I CRISPR-Cas System

P Yang, S Zhang, D Hu, X Li, Y Guo, H Guo… - International Journal of …, 2024 - mdpi.com
The CRISPR-Cas system functions as an adaptive immune mechanism in archaea and
bacteria, providing defense against the invasion of foreign nucleic acids. Most CRISPR-Cas …

RNA-based regulation in bacteria-phage interactions

M Saunier, LC Fortier, O Soutourina - Anaerobe, 2024 - Elsevier
Interactions of bacteria with their viruses named bacteriophages or phages shape the
bacterial genome evolution and contribute to the diversity of phages. RNAs have emerged …

The type IG CRISPR system: mechanism, structure and application

Q Shangguan - 2023 - research-repository.st-andrews.ac …
CRISPR is originally discovered as an adaptive immune system in prokaryotes. It has been
widely repurposed for application in different microbiological fields attributed to its ability to …