Deep learning for cellular image analysis

E Moen, D Bannon, T Kudo, W Graf, M Covert… - Nature …, 2019 - nature.com
Recent advances in computer vision and machine learning underpin a collection of
algorithms with an impressive ability to decipher the content of images. These deep learning …

Regulation of clathrin-mediated endocytosis

M Mettlen, PH Chen, S Srinivasan… - Annual review of …, 2018 - annualreviews.org
Clathrin-mediated endocytosis (CME) is the major endocytic pathway in mammalian cells. It
is responsible for the uptake of transmembrane receptors and transporters, for remodeling …

Minimal information for studies of extracellular vesicles (MISEV2023): From basic to advanced approaches

JA Welsh, DCI Goberdhan, L O'Driscoll… - Journal of …, 2024 - Wiley Online Library
Extracellular vesicles (EVs), through their complex cargo, can reflect the state of their cell of
origin and change the functions and phenotypes of other cells. These features indicate …

TrackMate 7: integrating state-of-the-art segmentation algorithms into tracking pipelines

D Ershov, MS Phan, JW Pylvänäinen, SU Rigaud… - Nature …, 2022 - nature.com
TrackMate is an automated tracking software used to analyze bioimages and is distributed
as a Fiji plugin. Here, we introduce a new version of TrackMate. TrackMate 7 is built to …

Condensed but liquid-like domain organization of active chromatin regions in living human cells

T Nozaki, S Shinkai, S Ide, K Higashi, S Tamura… - Science …, 2023 - science.org
In eukaryotes, higher-order chromatin organization is spatiotemporally regulated as
domains, for various cellular functions. However, their physical nature in living cells remains …

[HTML][HTML] TrackMate: An open and extensible platform for single-particle tracking

JY Tinevez, N Perry, J Schindelin, GM Hoopes… - Methods, 2017 - Elsevier
We present TrackMate, an open source Fiji plugin for the automated, semi-automated, and
manual tracking of single-particles. It offers a versatile and modular solution that works out of …

Applying systems-level spectral imaging and analysis to reveal the organelle interactome

AM Valm, S Cohen, WR Legant, J Melunis… - Nature, 2017 - nature.com
The organization of the eukaryotic cell into discrete membrane-bound organelles allows for
the separation of incompatible biochemical processes, but the activities of these organelles …

Single-molecule FRET imaging of GPCR dimers in living cells

WB Asher, P Geggier, MD Holsey, GT Gilmore… - Nature …, 2021 - nature.com
Class CG protein-coupled receptors (GPCRs) are known to form stable homodimers or
heterodimers critical for function, but the oligomeric status of class A and B receptors, which …

Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms

TL Liu, S Upadhyayula, DE Milkie, V Singh, K Wang… - Science, 2018 - science.org
INTRODUCTION Organisms live by means of the complex, dynamic, three-dimensional (3D)
interplay between millions of components, from the molecular to the multicellular. Visualizing …

Thermal skyrmion diffusion used in a reshuffler device

J Zázvorka, F Jakobs, D Heinze, N Keil… - Nature …, 2019 - nature.com
Magnetic skyrmions in thin films can be efficiently displaced with high speed by using spin-
transfer torques, and spin–orbit torques,–at low current densities. Although this favourable …