Mammalian genomes express two principal gene categories through RNA polymerase II-
mediated transcription: protein-coding transcription units and non-coding RNA transcription …

RNA–DNA hybrids are generated during transcription, DNA replication and DNA repair and
are crucial intermediates in these processes. When RNA–DNA hybrids are stably formed in …

Although transcription is an essential cellular process, it is paradoxically also a well-
recognized cause of genomic instability. R-loops, non-B DNA structures formed when …

R-loops are three-stranded structures that harbour an RNA–DNA hybrid and frequently form
during transcription. R-loop misregulation is associated with DNA damage, transcription …

During transcription, the nascent RNA strand can base pair with its template DNA, displacing
the non-template strand as ssDNA and forming a structure called an R-loop. R-loops are …

Double-strand breaks (DSBs) are the most deleterious DNA lesions, which, if left unrepaired,
may lead to genome instability or cell death. Here, we report that, in response to DSBs, the …

DNA-RNA hybrids play a physiological role in cellular processes, but often, they represent
non-scheduled co-transcriptional structures with a negative impact on transcription …

The journey of RNA polymerase II (Pol II) as it transcribes a gene is anything but a smooth
ride. Transcript elongation is discontinuous and can be perturbed by intrinsic regulatory …

The tumour suppressor breast cancer type 1 susceptibility protein (BRCA1) promotes DNA
double-strand break (DSB) repair by homologous recombination and protects DNA …

Centromeric integrity is key for proper chromosome segregation during cell division.
Centromeres have unique chromatin features that are essential for centromere …