High-resolution studies of lysis–lysogeny decision-making in bacteriophage lambda

Q Shao, JT Trinh, L Zeng - Journal of Biological Chemistry, 2019 - ASBMB
Cellular decision-making guides complex development such as cell differentiation and
disease progression. Much of our knowledge about decision-making is derived from simple …

Single-cell studies of phage λ: hidden treasures under Occam's rug

I Golding - Annual Review of Virology, 2016 - annualreviews.org
Studies over more than half a century have resulted in what some consider a complete
narrative for the life cycle of bacteriophage λ. However, this narrative is only complete within …

Using bacterial population dynamics to count phages and their lysogens

Y Geng, TVP Nguyen, E Homaee, I Golding - Nature communications, 2024 - nature.com
Traditional assays for counting bacteriophages and their lysogens are labor-intensive and
perturbative to the host cells. Here, we present a high-throughput infection method in a …

Temperature-induced DNA density transition in phage λ capsid revealed with contrast-matching SANS

JR Villanueva Valencia, E Tsimtsirakis… - Proceedings of the …, 2023 - National Acad Sciences
Structural details of a genome packaged in a viral capsid are essential for understanding
how the structural arrangement of a viral genome in a capsid controls its release dynamics …

CRP represses the CRISPR/Cas system in Escherichia coli: evidence that endogenous CRISPR spacers impede phage P1 replication

CD Yang, YH Chen, HY Huang… - Molecular …, 2014 - Wiley Online Library
The CRISPR/Cas system is an important aspect in bacterial immunology. The anti‐phage
activity of the CRISPR system has been established using synthetic CRISPR spacers, but in …

Bacteriophages from ExPEC reservoirs kill pandemic multidrug-resistant strains of clonal group ST131 in animal models of bacteremia

SI Green, JT Kaelber, L Ma, BW Trautner, RF Ramig… - Scientific Reports, 2017 - nature.com
Multi-drug resistant (MDR) enteric bacteria are of increasing global concern. A clonal group,
Escherichia coli sequence type (ST) 131, harbors both MDR and a deadly complement of …

Stable-isotope probing and metagenomics reveal predation by protozoa drives E. coli removal in slow sand filters

SJ Haig, M Schirmer, R D'Amore, J Gibbs… - The ISME …, 2015 - academic.oup.com
Stable-isotope probing and metagenomics were applied to study samples taken from
laboratory-scale slow sand filters 0.5, 1, 2, 3 and 4 h after challenging with 13C-labelled …

K5 Capsule and Lipopolysaccharide Are Important in Resistance to T4 Phage Attack in Probiotic E. coli Strain Nissle 1917

M Soundararajan, R von Bünau… - Frontiers in …, 2019 - frontiersin.org
Rapidly growing antibiotic resistance among gastrointestinal pathogens, and the ability of
antibiotics to induce the virulence of these pathogens makes it increasingly difficult to rely on …

Coinfecting phages impede each other's entry into the cell

TVP Nguyen, Y Wu, T Yao, JT Trinh, L Zeng… - Current Biology, 2024 - cell.com
The developmental choice made by temperate phages, between cell death (lysis) and viral
dormancy (lysogeny), is influenced by the relative abundance of viruses and hosts in the …

Lysis‐lysogeny coexistence: prophage integration during lytic development

Q Shao, JT Trinh, CS McIntosh, B Christenson… - …, 2017 - Wiley Online Library
The infection of Escherichia coli cells by bacteriophage lambda results in bifurcated means
of propagation, where the phage decides between the lytic and lysogenic pathways …