Spatiotemporal control of CRISPR/Cas9 gene editing
The clustered regularly interspaced short palindromic repeats (CRISPR)/associated protein
9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic …
9 (CRISPR/Cas9) gene editing technology, as a revolutionary breakthrough in genetic …
Activation of mechanoluminescent nanotransducers by focused ultrasound enables light delivery to deep-seated tissue in vivo
Light is used extensively in biological and medical research for optogenetic
neuromodulation, fluorescence imaging, photoactivatable gene editing and light-based …
neuromodulation, fluorescence imaging, photoactivatable gene editing and light-based …
Photocontrolled crRNA activation enables robust CRISPR-Cas12a diagnostics
M Hu, Z Qiu, Z Bi, T Tian, Y Jiang… - Proceedings of the …, 2022 - National Acad Sciences
CRISPR diagnostics based on nucleic acid amplification faces barriers to its commercial
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …
use, such as contamination risks and insufficient sensitivity. Here, we propose a robust …
Photoactivatable CRISPR/Cas12a strategy for one-pot DETECTR molecular diagnosis
Y Chen, X Xu, J Wang, Y Zhang, W Zeng… - Analytical …, 2022 - ACS Publications
As a golden partner of recombinase polymerase amplification (RPA), CRISPR/Cas12a has
been proven to solve the false-positive problem caused by nonspecific amplification …
been proven to solve the false-positive problem caused by nonspecific amplification …
Engineered materials for in vivo delivery of genome-editing machinery
Genome-editing technologies, such as CRISPR–Cas9, are promising for treating otherwise
incurable genetic diseases. Great progress has been made for ex vivo genome editing; …
incurable genetic diseases. Great progress has been made for ex vivo genome editing; …
Guiding lights: tissue bioprinting using photoactivated materials
Photoactivated materials have found widespread use in biological and medical applications
and are playing an increasingly important role in the nascent field of three-dimensional (3D) …
and are playing an increasingly important role in the nascent field of three-dimensional (3D) …
Optogenetic control of RNA function and metabolism using engineered light-switchable RNA-binding proteins
R Liu, J Yang, J Yao, Z Zhao, W He, N Su… - Nature …, 2022 - nature.com
RNA-binding proteins (RBPs) play an essential role in regulating the function of RNAs in a
cellular context, but our ability to control RBP activity in time and space is limited. Here, we …
cellular context, but our ability to control RBP activity in time and space is limited. Here, we …
Genome editing in bacteria: CRISPR-Cas and beyond
RD Arroyo-Olarte, R Bravo Rodriguez, E Morales-Ríos - Microorganisms, 2021 - mdpi.com
Genome editing in bacteria encompasses a wide array of laborious and multi-step methods
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
such as suicide plasmids. The discovery and applications of clustered regularly interspaced …
Cardiac tissue engineering: state-of-the-art methods and outlook
AH Nguyen, P Marsh, L Schmiess-Heine… - Journal of biological …, 2019 - Springer
The purpose of this review is to assess the state-of-the-art fabrication methods, advances in
genome editing, and the use of machine learning to shape the prospective growth in cardiac …
genome editing, and the use of machine learning to shape the prospective growth in cardiac …
Optogenetics for transcriptional programming and genetic engineering
Optogenetics combines genetics and biophotonics to enable noninvasive control of
biological processes with high spatiotemporal precision. When engineered into protein …
biological processes with high spatiotemporal precision. When engineered into protein …