Over the past decade, RNA sequencing (RNA-seq) has become an indispensable tool for
transcriptome-wide analysis of differential gene expression and differential splicing of …

Single-cell RNA sequencing (scRNA-seq) is a popular and powerful technology that allows
you to profile the whole transcriptome of a large number of individual cells. However, the …

Methods for profiling genes at the single-cell level have revolutionized our ability to study
several biological processes and systems including development, differentiation, response …

Background Droplet-based single-cell RNA sequence analyses assume that all acquired
RNAs are endogenous to cells. However, any cell-free RNAs contained within the input …

Hepatocellular carcinoma (HCC) represents a paradigm of the relation between tumor
microenvironment (TME) and tumor development. Here, we generate a single-cell atlas of …

Background Next-generation sequencing (NGS) has made it possible to determine the
sequence and relative abundance of all nucleotides in a biological or environmental …

Conventional human embryonic stem cells are considered to be primed pluripotent but can
be induced to enter a naive state. However, the transcriptional features associated with …

Single-cell RNA sequencing (scRNA-seq) has opened new avenues for the characterization
of heterogeneity in a large variety of cellular systems. As this is a relatively new technique …

Data normalization is vital to single-cell sequencing, addressing limitations presented by low
input material and various forms of bias or noise present in the sequencing process. Several …

Single-cell RNA-seq reveals the role of pathogenic cell populations in development and
progression of chronic diseases. In order to expand our knowledge on cellular …