MiFish metabarcoding: a high-throughput approach for simultaneous detection of multiple fish species from environmental DNA and other samples
We reviewed the current methodology and practices of the DNA metabarcoding approach
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …
using a universal PCR primer pair MiFish, which co-amplifies a short fragment of fish DNA …
Lake sedimentary DNA research on past terrestrial and aquatic biodiversity: Overview and recommendations
The use of lake sedimentary DNA to track the long-term changes in both terrestrial and
aquatic biota is a rapidly advancing field in paleoecological research. Although largely …
aquatic biota is a rapidly advancing field in paleoecological research. Although largely …
Navigating the seven challenges of taxonomic reference databases in metabarcoding analyses
Assessment of biodiversity using metabarcoding data, such as from bulk or environmental
DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and …
DNA sampling, is becoming increasingly relevant in ecology, biodiversity sciences and …
eDNA metabarcoding as a biomonitoring tool for marine protected areas
Z Gold, J Sprague, DJ Kushner, E Zerecero Marin… - PLoS …, 2021 - journals.plos.org
Monitoring of marine protected areas (MPAs) is critical for marine ecosystem management,
yet current protocols rely on SCUBA-based visual surveys that are costly and time …
yet current protocols rely on SCUBA-based visual surveys that are costly and time …
The expanding role of DNA barcodes: Indispensable tools for ecology, evolution, and conservation
DNA barcoding has transformed the fields of ecology, evolution, and conservation by
providing a rapid and effective tool for species identification. The growth of DNA barcodes as …
providing a rapid and effective tool for species identification. The growth of DNA barcodes as …
Improving metabarcoding taxonomic assignment: A case study of fishes in a large marine ecosystem
DNA metabarcoding is an important tool for molecular ecology. However, its effectiveness
hinges on the quality of reference sequence databases and classification parameters …
hinges on the quality of reference sequence databases and classification parameters …
A pile of pipelines: An overview of the bioinformatics software for metabarcoding data analyses
A Hakimzadeh, A Abdala Asbun… - Molecular Ecology …, 2024 - Wiley Online Library
Environmental DNA (eDNA) metabarcoding has gained growing attention as a strategy for
monitoring biodiversity in ecology. However, taxa identifications produced through …
monitoring biodiversity in ecology. However, taxa identifications produced through …
eDNA captures depth partitioning in a kelp forest ecosystem
Environmental DNA (eDNA) metabarcoding is an increasingly important tool for surveying
biodiversity in marine ecosystems. However, the scale of temporal and spatial variability in …
biodiversity in marine ecosystems. However, the scale of temporal and spatial variability in …
Benchmarking bioinformatic tools for fast and accurate eDNA metabarcoding species identification
L Mathon, A Valentini, PE Guérin… - Molecular Ecology …, 2021 - Wiley Online Library
Bioinformatic analysis of eDNA metabarcoding data is a crucial step toward rigorously
assessing biodiversity. Many programs are now available for each step of the required …
assessing biodiversity. Many programs are now available for each step of the required …
eDNAFlow, an automated, reproducible and scalable workflow for analysis of environmental DNA sequences exploiting Nextflow and Singularity
M Mousavi‐Derazmahalleh, A Stott… - Molecular Ecology …, 2021 - Wiley Online Library
Metabarcoding of environmental DNA (eDNA) when coupled with high throughput
sequencing is revolutionising the way biodiversity can be monitored across a wide range of …
sequencing is revolutionising the way biodiversity can be monitored across a wide range of …