Bacillus subtilis is a widely distributed aerobic Gram-positive species of bacteria. As a tool in
the lab, it has the advantages of nonpathogenicity and limited likelihood of becoming drug …

Bacillus subtilis spore surface display (BSSD) technology is considered to be one of the
most promising approaches for expressing heterologous proteins with high activity and …

Efforts to improve CRISPR-Cas9 genome editing systems for lower off-target effects are
mostly at the cost of its robust on-target efficiency. To enhance both accuracy and efficiency …

Cell surface display of heavy metal‐binding proteins has been used to enhance the
adsorption capacity of heavy metals and the engineered microbial cells can be potentially …

Geranyl acetate is, as one of the monoterpenoids, a natural constituent of more than 60
essential oils. Production of such an ester fragrance compound by plant extracts is limited by …

Protein fusion using a linker plays an important role for protein evolution. However,
designing suitable linkers for protein evolution is yet challenging and under-explored. To …

Spring viremia of carp virus (SVCV) is a causative agent which causes great economic
losses to freshwater aquaculture industries. For the prevention and control of viral diseases …

In fusion protein design strategies, the flexibility and length of linkers are important
parameters affecting the bioactivity of multifunctional proteins. A series of fusion proteins …

A rational design method to improve β-mannanase (ManTJ102) thermostability was
developed successfully in this study. The flexible area of residues 330–340 in ManTJ102 …

The production of recombinant proteins in the microbial host Escherichia coli often results in
the formation of cytoplasmic protein inclusion bodies (IBs). Proteins forming IBs are often …