Microbial production of advanced biofuels

J Keasling, H Garcia Martin, TS Lee… - Nature Reviews …, 2021 - nature.com
Concerns over climate change have necessitated a rethinking of our transportation
infrastructure. One possible alternative to carbon-polluting fossil fuels is biofuels produced …

The emergence of adaptive laboratory evolution as an efficient tool for biological discovery and industrial biotechnology

TE Sandberg, MJ Salazar, LL Weng, BO Palsson… - Metabolic …, 2019 - Elsevier
Harnessing the process of natural selection to obtain and understand new microbial
phenotypes has become increasingly possible due to advances in culturing techniques …

Metabolic burden: cornerstones in synthetic biology and metabolic engineering applications

G Wu, Q Yan, JA Jones, YJ Tang, SS Fong… - Trends in …, 2016 - cell.com
Engineering cell metabolism for bioproduction not only consumes building blocks and
energy molecules (eg, ATP) but also triggers energetic inefficiency inside the cell. The …

Constraining the metabolic genotype–phenotype relationship using a phylogeny of in silico methods

NE Lewis, H Nagarajan, BO Palsson - Nature Reviews Microbiology, 2012 - nature.com
Reconstructed microbial metabolic networks facilitate a mechanistic description of the
genotype–phenotype relationship through the deployment of constraint-based …

Systems metabolic engineering of microorganisms for natural and non-natural chemicals

JW Lee, D Na, JM Park, J Lee, S Choi… - Nature chemical biology, 2012 - nature.com
Growing concerns over limited fossil resources and associated environmental problems are
motivating the development of sustainable processes for the production of chemicals, fuels …

The biomass objective function

AM Feist, BO Palsson - Current opinion in microbiology, 2010 - Elsevier
Flux balance analysis (FBA) is a mathematical approach for analyzing the flow of
metabolites through a metabolic network. To computationally predict cell growth using FBA …

[HTML][HTML] Automating the design-build-test-learn cycle towards next-generation bacterial cell factories

N Gurdo, DC Volke, D McCloskey, PI Nikel - New Biotechnology, 2023 - Elsevier
Automation is playing an increasingly significant role in synthetic biology. Groundbreaking
technologies, developed over the past 20 years, have enormously accelerated the …

Synthesis and Accumulation of Aromatic Aldehydes in an Engineered Strain of Escherichia coli

AM Kunjapur, Y Tarasova… - Journal of the American …, 2014 - ACS Publications
Aromatic aldehydes are useful in numerous applications, especially as flavors, fragrances,
and pharmaceutical precursors. However, microbial synthesis of aldehydes is hindered by …

Design and analysis of synthetic carbon fixation pathways

A Bar-Even, E Noor, NE Lewis… - Proceedings of the …, 2010 - National Acad Sciences
Carbon fixation is the process by which CO2 is incorporated into organic compounds. In
modern agriculture in which water, light, and nutrients can be abundant, carbon fixation …

Reconstruction of biochemical networks in microorganisms

AM Feist, MJ Herrgård, I Thiele, JL Reed… - Nature Reviews …, 2009 - nature.com
Abstract Systems analysis of metabolic and growth functions in microbial organisms is
rapidly developing and maturing. Such studies are enabled by reconstruction, at the …