Optical spectroscopy for in vivo medical diagnosis—a review of the state of the art and future perspectives

JA Kim, DJ Wales, GZ Yang - Progress in Biomedical …, 2020 - iopscience.iop.org
When light is incident to a biological tissue surface, combinations of optical processes occur,
such as reflection, absorption, elastic and non-elastic scattering, and fluorescence. Analysis …

Mesoscopic fluorescence lifetime imaging: Fundamental principles, clinical applications and future directions

A Alfonso‐Garcia, J Bec, B Weyers… - Journal of …, 2021 - Wiley Online Library
Fluorescence lifetime imaging (FLIm) is an optical spectroscopic imaging technique capable
of real‐time assessments of tissue properties in clinical settings. Label‐free FLIm is sensitive …

The docking of synaptic vesicles on the presynaptic membrane induced by α-synuclein is modulated by lipid composition

WK Man, B Tahirbegi, MD Vrettas, S Preet… - Nature …, 2021 - nature.com
Abstract α-Synuclein (αS) is a presynaptic disordered protein whose aberrant aggregation is
associated with Parkinson's disease. The functional role of αS is still debated, although it …

Intraoperative margin assessment in oral and oropharyngeal cancer using label-free fluorescence lifetime imaging and machine learning

M Marsden, BW Weyers, J Bec, T Sun… - IEEE Transactions …, 2020 - ieeexplore.ieee.org
Objective: To demonstrate the diagnostic ability of label-free, point-scanning, fiber-based
Fluorescence Lifetime Imaging (FLIm) as a means of intraoperative guidance during oral …

Investigations on average fluorescence lifetimes for visualizing multi-exponential decays

Y Li, S Natakorn, Y Chen, M Safar… - Frontiers in …, 2020 - frontiersin.org
Intensity-and amplitude-weighted average lifetimes, denoted as τ I and τ A hereafter, are
useful indicators for revealing Förster resonance energy transfer (FRET) or fluorescence …

Autofluorescence lifetime augmented reality as a means for real-time robotic surgery guidance in human patients

D Gorpas, J Phipps, J Bec, D Ma, S Dochow… - Scientific reports, 2019 - nature.com
Due to loss of tactile feedback the assessment of tumor margins during robotic surgery is
based only on visual inspection, which is neither significantly sensitive nor specific. Here we …

Real-time diagnosis and visualization of tumor margins in excised breast specimens using fluorescence lifetime imaging and machine learning

J Unger, C Hebisch, JE Phipps, JL Lagarto… - Biomedical optics …, 2020 - opg.optica.org
Tumor-free surgical margins are critical in breast-conserving surgery. In up to 38% of the
cases, however, patients undergo a second surgery since malignant cells are found at the …

Real‐time augmented reality for delineation of surgical margins during neurosurgery using autofluorescence lifetime contrast

A Alfonso‐Garcia, J Bec… - Journal of …, 2020 - Wiley Online Library
Current clinical brain imaging techniques used for surgical planning of tumor resection lack
intraoperative and real‐time feedback; hence surgeons ultimately rely on subjective …

Design and evaluation of a device for fast multispectral time-resolved fluorescence spectroscopy and imaging

DR Yankelevich, D Ma, J Liu, Y Sun, Y Sun… - Review of Scientific …, 2014 - pubs.aip.org
The application of time-resolved fluorescence spectroscopy (TRFS) to in vivo tissue
diagnosis requires a method for fast acquisition of fluorescence decay profiles in multiple …

Endoscopic Fluorescence Lifetime Imaging for In Vivo Intraoperative Diagnosis of Oral Carcinoma

Y Sun, JE Phipps, J Meier, N Hatami… - Microscopy and …, 2013 - academic.oup.com
A clinically compatible fluorescence lifetime imaging microscopy (FLIM) system was
developed. The system was applied to intraoperative in vivo imaging of head and neck …