During pre-mRNA splicing, exons in the primary transcript are precisely connected to
generate an mRNA. Intron lariat RNAs are formed as by-products of this process. In addition …

Post-transcriptional control of mRNA trafficking and metabolism plays a critical role in the
actualization and fine tuning of the genetic program of cells, both in development and in …

Total RNA sequencing has been used to reveal poly (A) and non-poly (A) RNA expression,
RNA processing and enhancer activity. To date, no method for full-length total RNA …

Introns are ubiquitous in eukaryotic genomes and have long been considered as 'junk
RNA'but the huge energy expenditure in their transcription, removal, and degradation …

Introns represent almost half of the human genome, yet their vast majority is eliminated from
eukaryotic transcripts through RNA splicing. Nevertheless, they feature key elements and …

The dystrophin protein encoding DMD gene is the longest human gene. The 2.2 Mb long
human dystrophin transcript takes 16 hours to be transcribed and is co-transcriptionally …

Splicing of pre-mRNA is a crucial regulatory stage in the pathway of gene expression
controlled by multiple post-and co-transcriptional mechanisms. The large Duchenne …

Abstract Circular RNAs (CircRNAs) are a sub-class of non-coding RNA, which are covalently
closed at the ends through a non-canonical process called, backsplicing from the precursor …

Tools to understand how the spliceosome functions in vivo have lagged behind advances in
the structural biology of the spliceosome. Here, methods are described to globally profile …

Duchenne muscular dystrophy (DMD) is a severe muscular disorder. It was reported that
multiple exon skipping (MES), targeting exon 45–55 of the DMD gene, might improve …