Self-assembling nucleic acid nanostructures functionalized with aptamers

A Krissanaprasit, CM Key, S Pontula… - Chemical …, 2021 - ACS Publications
Researchers have worked for many decades to master the rules of biomolecular design that
would allow artificial biopolymer complexes to self-assemble and function similarly to the …

Transfer RNA recognition by aminoacyl‐tRNA synthetases

PJ Beuning, K Musier‐Forsyth - … : Original Research on …, 1999 - Wiley Online Library
The aminoacyl‐tRNA synthetases are an ancient group of enzymes that catalyze the
covalent attachment of an amino acid to its cognate transfer RNA. The question of specificity …

[HTML][HTML] Class I tyrosyl‐tRNA synthetase has a class II mode of cognate tRNA recognition

A Yaremchuk, I Kriklivyi, M Tukalo, S Cusack - The EMBO journal, 2002 - embopress.org
Bacterial tyrosyl‐tRNA synthetases (TyrRS) possess a flexibly linked C‐terminal domain of∼
80 residues, which has hitherto been disordered in crystal structures of the enzyme. We …

Square-shaped RNA particles from different RNA folds

I Severcan, C Geary, E Verzemnieks, A Chworos… - Nano …, 2009 - ACS Publications
The structural information encoding specific conformations of natural RNAs can be
implemented within artificial RNA sequences to control both three-dimensional (3D) shape …

Chemical and enzymatic synthesis of tRNAs for high-throughput crystallization

LD Sherlin, TL Bullock, TA Nissan, JJ Perona… - Rna, 2001 - cambridge.org
Preparation of large quantities of RNA molecules of a defined sequence is a prerequisite for
biophysical analysis, and is particularly important to the determination of high-resolution …

Cooperative effects by the initiation codon and its flanking regions on translation initiation

CM Stenström, E Holmgren, LA Isaksson - Gene, 2001 - Elsevier
The purine-rich Shine–Dalgarno (SD) sequence located a few bases upstream of the mRNA
initiation codon supports translation initiation by complementary binding to the anti-SD in the …

Amino acid discrimination by a class I aminoacyl-tRNA synthetase specified by negative determinants

TL Bullock, N Uter, TA Nissan, JJ Perona - Journal of molecular biology, 2003 - Elsevier
The 2.5 Å crystal structure of Escherichia coli glutaminyl-tRNA synthetase in a quaternary
complex with tRNAGln, an ATP analog and glutamate reveals that the non-cognate amino …

tRNA-dependent aminoacyl-adenylate hydrolysis by a nonediting class I aminoacyl-tRNA synthetase

I Gruic-Sovulj, N Uter, T Bullock, JJ Perona - Journal of Biological Chemistry, 2005 - ASBMB
Glutaminyl-tRNA synthetase generates Gln-tRNA Gln 10 7-fold more efficiently than Glu-
tRNA Gln and requires tRNA to synthesize the activated aminoacyl adenylate in the first step …

Tertiary core rearrangements in a tight binding transfer RNA aptamer

TL Bullock, LD Sherlin, JJ Perona - Nature structural biology, 2000 - nature.com
Guided by an in vitro selection experiment designed to obtain tight binding aptamers of
Escherichia coli glutamine specific tRNA (tRNA Gln) for glutaminyl-tRNA synthetase …

tRNA leucine identity and recognition sets

G Tocchini-Valentini, ME Saks, J Abelson - Journal of molecular biology, 2000 - Elsevier
Transfer RNAs (tRNAs) are grouped into two classes based on the structure of their variable
loop. In Escherichia coli, tRNAs from three isoaccepting groups are classified as type II …