Long-lived emissive probes for time-resolved photoluminescence bioimaging and biosensing

KY Zhang, Q Yu, H Wei, S Liu, Q Zhao… - Chemical …, 2018 - ACS Publications
In this Review article, we systematically summarize the design and applications of various
kinds of long-lived emissive probes for bioimaging and biosensing via time-resolved …

FRAP, FLIM, and FRET: Detection and analysis of cellular dynamics on a molecular scale using fluorescence microscopy

C De Los Santos, CW Chang… - Molecular …, 2015 - Wiley Online Library
The combination of fluorescent‐probe technology plus modern optical microscopes allows
investigators to monitor dynamic events in living cells with exquisite temporal and spatial …

Assessing sampling interval-dependent roughness in fractured steel fiber reinforced concrete using a double-exponential decay model

S Hou, K Li, X Hu, C Shi - Cement and Concrete Composites, 2024 - Elsevier
This study introduces a double-exponential decay (DED) model to investigate the sampling
interval (δ)-dependent behavior of crack morphology roughness in steel fiber reinforced …

Enhancing precision in time-domain fluorescence lifetime imaging

CW Chang, MA Mycek - Journal of biomedical optics, 2010 - spiedigitallibrary.org
In biological applications of fluorescence lifetime imaging, low signals from samples can be
a challenge, causing poor lifetime precision. We demonstrate how optimal signal gating (a …

[HTML][HTML] Can we use rapid lifetime determination for fast, fluorescence lifetime based, metabolic imaging? Precision and accuracy of double-exponential decay …

SF Silva, JP Domingues, AM Morgado - Plos one, 2019 - journals.plos.org
Fluorescence lifetime imaging microscopy (FLIM) can assess cell's metabolism through the
fluorescence of the co-enzymes NADH and FAD, which exhibit a double-exponential decay …

Precise fluorophore lifetime mapping in live-cell, multi-photon excitation microscopy

CW Chang, MA Mycek - Optics Express, 2010 - opg.optica.org
Fluorophore excited state lifetime is a useful indicator of micro-environment in cellular
optical molecular imaging. For quantitative sensing, precise lifetime determination is …

Time-gated fluorescence lifetime microscope with light-sheet illumination

AÍD Machado - 2021 - estudogeral.uc.pt
Time-Gated fluorescence lifetime microscopy is based on the fluorescence decay process
and allows to observe the spatial distribution of the fluorophores contained in a sample …

Increasing precision of lifetime determination in fluorescence lifetime imaging

CW Chang, MA Mycek - Three-Dimensional and …, 2010 - spiedigitallibrary.org
The interest in fluorescence lifetime imaging microscopy (FLIM) is increasing, as commercial
FLIM modules become available for confocal and multi-photon microscopy. In biological …

Time-Gated Fluorescence Lifetime Microscopy Methods and Instrumentation for Metabolic Imaging

SF Silva - 2019 - estudogeral.uc.pt
Time-Gated fluorescence lifetime imaging microscopy (FLIM) is a microscopy technique
where fluorescence decay curves are acquired by opening a series of time windows …

Laser induced chlorophyll fluorescence lifetime measurement and characteristic analysis for plant drought-stress

W Wenbo, H Dengxin, L Jing… - 2013 IEEE 11th …, 2013 - ieeexplore.ieee.org
A novel fluorescence lidar technique using plant fluorescence lifetime is presented to
evaluate the living status for plant growth, the environmental monitoring, and so on. A 532 …