This work describes the direct hyphenation of cation exchange chromatography (CEX) with
a compact, easy-to-use benchtop Time of Flight mass spectrometer (ToF/MS) for the …

This review focuses on the deamidation of asparagine in monoclonal antibodies, one of the
well-known degradation pathways. The importance of close monitoring of deamidation from …

Although the field of antibody drugs has grown larger, the antibody production still faces
several challenges. Effective antibody purification is required, but the conventional …

Monoclonal antibodies (mAbs) are heterogeneous macromolecules that display a complex
isoform profile as a result of the large series of modifications they can undergo. Product …

The characterization of proteins and complexes in biological systems is essential to
establish their critical properties and to understand their unique functions in a plethora of …

In ionexchange chromatography, the pH gradient mode becomes more and more popular
today for the analysis of therapeutic proteins as this mode can provide higher or alternative …

Monoclonal antibodies (mAbs) are an essential class of biotherapeutics. A platform process
is used for mAb development to ensure clinically safe and stable molecules. Regulatory …

Immunoglobulin G (IgG) is the most common monoclonal antibody (mAb) grown for
therapeutic applications. While IgG is often selectively isolated from cell lines using protein A …

This study details the development of on-line two-dimensional liquid chromatography (2D-
LC) methods combining cation-exchange chromatography (CEX) and reversed-phase liquid …

Arginine is the essential amino acid having potential therapeutic value. The amino acid can
be extracted from different natural resources. But they are not feasible due to efficient …