A primer on metagenomics
JC Wooley, A Godzik, I Friedberg - PLoS computational biology, 2010 - journals.plos.org
Metagenomics is a discipline that enables the genomic study of uncultured microorganisms.
Faster, cheaper sequencing technologies and the ability to sequence uncultured microbes …
Faster, cheaper sequencing technologies and the ability to sequence uncultured microbes …
Development of clade-specific Symbiodinium primers for quantitative PCR (qPCR) and their application to detecting clade D symbionts in Caribbean corals
We developed quantitative PCR (qPCR) assays to distinguish each of the four clades (A–D)
of dinoflagellate endosymbionts (genus Symbiodinium) commonly found in Caribbean …
of dinoflagellate endosymbionts (genus Symbiodinium) commonly found in Caribbean …
Building consensus around the assessment and interpretation of Symbiodiniaceae diversity
Dinoflagellates in the family Symbiodiniaceae occupy multiple ecological niches on tropical,
subtropical, and temperate reefs, ranging from species that are exclusively free-living to …
subtropical, and temperate reefs, ranging from species that are exclusively free-living to …
Specificity is rarely absolute in coral–algal symbiosis: implications for coral response to climate change
RN Silverstein, AMS Correa… - Proceedings of the …, 2012 - royalsocietypublishing.org
Some reef-building corals have been shown to respond to environmental change by shifting
the composition of their algal symbiont (genus Symbiodinium) communities. These shifts …
the composition of their algal symbiont (genus Symbiodinium) communities. These shifts …
Quantification of diatom and dinoflagellate biomasses in coastal marine seawater samples by real-time PCR
A Godhe, ME Asplund, K Härnström… - Applied and …, 2008 - Am Soc Microbiol
Two real-time PCR assays targeting the small-subunit (SSU) ribosomal DNA (rDNA) were
designed to assess the proportional biomass of diatoms and dinoflagellates in marine …
designed to assess the proportional biomass of diatoms and dinoflagellates in marine …
Symbiodinium spp. in colonies of eastern Pacific Pocillopora spp. are highly stable despite the prevalence of low-abundance background populations
MP McGinley, MD Aschaffenburg, DT Pettay… - Marine Ecology …, 2012 - int-res.com
A shift in the dominant Symbiodinium species within a coral colony may allow rapid
acclimatization to environmental stress, provided that the new symbiont is better suited to …
acclimatization to environmental stress, provided that the new symbiont is better suited to …
Quantification of algal endosymbionts (Symbiodinium) in coral tissue using real‐time PCR
JC Mieog, MJH van Oppen… - Molecular Ecology …, 2009 - Wiley Online Library
Understanding the flexibility of the endosymbioses between scleractinian corals and single‐
cell algae of the genus Symbiodinium will provide valuable insights into the future of coral …
cell algae of the genus Symbiodinium will provide valuable insights into the future of coral …
Functional significance of genetically different symbiotic algae Symbiodinium in a coral reef symbiosis
JE Loram, HG Trapido‐Rosenthal… - Molecular …, 2007 - Wiley Online Library
The giant sea anemone Condylactis gigantea associates with members of two clades of the
dinoflagellate alga Symbiodinium, either singly or in mixed infection, as revealed by clade …
dinoflagellate alga Symbiodinium, either singly or in mixed infection, as revealed by clade …
Rapid, Precise, and Accurate Counts of Symbiodinium Cells Using the Guava Flow Cytometer, and a Comparison to Other Methods
CJ Krediet, JC DeNofrio, C Caruso, MS Burriesci… - PLoS …, 2015 - journals.plos.org
In studies of both the establishment and breakdown of cnidarian-dinoflagellate symbiosis, it
is often necessary to determine the number of Symbiodinium cells relative to the quantity of …
is often necessary to determine the number of Symbiodinium cells relative to the quantity of …
Genome size, rDNA copy, and qPCR assays for Symbiodiniaceae
Symbiodiniaceae community structure in corals is crucial for understanding the plasticity of
different holobionts under environmental stress. While this relies on molecular analyses …
different holobionts under environmental stress. While this relies on molecular analyses …