A primer on metagenomics

JC Wooley, A Godzik, I Friedberg - PLoS computational biology, 2010 - journals.plos.org
Metagenomics is a discipline that enables the genomic study of uncultured microorganisms.
Faster, cheaper sequencing technologies and the ability to sequence uncultured microbes …

Development of clade-specific Symbiodinium primers for quantitative PCR (qPCR) and their application to detecting clade D symbionts in Caribbean corals

AMS Correa, MD McDonald, AC Baker - Marine Biology, 2009 - Springer
We developed quantitative PCR (qPCR) assays to distinguish each of the four clades (A–D)
of dinoflagellate endosymbionts (genus Symbiodinium) commonly found in Caribbean …

Building consensus around the assessment and interpretation of Symbiodiniaceae diversity

SW Davies, MH Gamache, LI Howe-Kerr, NG Kriefall… - PeerJ, 2023 - peerj.com
Dinoflagellates in the family Symbiodiniaceae occupy multiple ecological niches on tropical,
subtropical, and temperate reefs, ranging from species that are exclusively free-living to …

Specificity is rarely absolute in coral–algal symbiosis: implications for coral response to climate change

RN Silverstein, AMS Correa… - Proceedings of the …, 2012 - royalsocietypublishing.org
Some reef-building corals have been shown to respond to environmental change by shifting
the composition of their algal symbiont (genus Symbiodinium) communities. These shifts …

Quantification of diatom and dinoflagellate biomasses in coastal marine seawater samples by real-time PCR

A Godhe, ME Asplund, K Härnström… - Applied and …, 2008 - Am Soc Microbiol
Two real-time PCR assays targeting the small-subunit (SSU) ribosomal DNA (rDNA) were
designed to assess the proportional biomass of diatoms and dinoflagellates in marine …

Symbiodinium spp. in colonies of eastern Pacific Pocillopora spp. are highly stable despite the prevalence of low-abundance background populations

MP McGinley, MD Aschaffenburg, DT Pettay… - Marine Ecology …, 2012 - int-res.com
A shift in the dominant Symbiodinium species within a coral colony may allow rapid
acclimatization to environmental stress, provided that the new symbiont is better suited to …

Quantification of algal endosymbionts (Symbiodinium) in coral tissue using real‐time PCR

JC Mieog, MJH van Oppen… - Molecular Ecology …, 2009 - Wiley Online Library
Understanding the flexibility of the endosymbioses between scleractinian corals and single‐
cell algae of the genus Symbiodinium will provide valuable insights into the future of coral …

Functional significance of genetically different symbiotic algae Symbiodinium in a coral reef symbiosis

JE Loram, HG Trapido‐Rosenthal… - Molecular …, 2007 - Wiley Online Library
The giant sea anemone Condylactis gigantea associates with members of two clades of the
dinoflagellate alga Symbiodinium, either singly or in mixed infection, as revealed by clade …

Rapid, Precise, and Accurate Counts of Symbiodinium Cells Using the Guava Flow Cytometer, and a Comparison to Other Methods

CJ Krediet, JC DeNofrio, C Caruso, MS Burriesci… - PLoS …, 2015 - journals.plos.org
In studies of both the establishment and breakdown of cnidarian-dinoflagellate symbiosis, it
is often necessary to determine the number of Symbiodinium cells relative to the quantity of …

Genome size, rDNA copy, and qPCR assays for Symbiodiniaceae

OS Saad, X Lin, TY Ng, L Li, P Ang, S Lin - Frontiers in Microbiology, 2020 - frontiersin.org
Symbiodiniaceae community structure in corals is crucial for understanding the plasticity of
different holobionts under environmental stress. While this relies on molecular analyses …