Light-sheets and smart microscopy, an exciting future is dawning
S Daetwyler, RP Fiolka - Communications biology, 2023 - nature.com
Light-sheet fluorescence microscopy has transformed our ability to visualize and
quantitatively measure biological processes rapidly and over long time periods. In this …
quantitatively measure biological processes rapidly and over long time periods. In this …
Practical considerations for quantitative light sheet fluorescence microscopy
Fluorescence microscopy has evolved from a purely observational tool to a platform for
quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic …
quantitative, hypothesis-driven research. As such, the demand for faster and less phototoxic …
Resolution doubling in light-sheet microscopy via oblique plane structured illumination
Structured illumination microscopy (SIM) doubles the spatial resolution of a fluorescence
microscope without requiring high laser powers or specialized fluorophores. However, the …
microscope without requiring high laser powers or specialized fluorophores. However, the …
[HTML][HTML] Two-photon synthetic aperture microscopy for minimally invasive fast 3D imaging of native subcellular behaviors in deep tissue
Holistic understanding of physio-pathological processes requires noninvasive 3D imaging in
deep tissue across multiple spatial and temporal scales to link diverse transient subcellular …
deep tissue across multiple spatial and temporal scales to link diverse transient subcellular …
Imagining the future of optical microscopy: everything, everywhere, all at once
H Balasubramanian, CM Hobson, TL Chew… - Communications …, 2023 - nature.com
The optical microscope has revolutionized biology since at least the 17th Century. Since
then, it has progressed from a largely observational tool to a powerful bioanalytical platform …
then, it has progressed from a largely observational tool to a powerful bioanalytical platform …
Engineered organoids for biomedical applications
As miniaturized and simplified stem cell-derived 3D organ-like structures, organoids are
rapidly emerging as powerful tools for biomedical applications. With their potential for …
rapidly emerging as powerful tools for biomedical applications. With their potential for …
Low-cost and scalable projected light-sheet microscopy for the high-resolution imaging of cleared tissue and living samples
Light-sheet fluorescence microscopy (LSFM) is a widely used technique for imaging cleared
tissue and living samples. However, high-performance LSFM systems are typically …
tissue and living samples. However, high-performance LSFM systems are typically …
Artificial confocal microscopy for deep label-free imaging
Wide-field microscopy of optically thick specimens typically features reduced contrast due to
spatial cross-talk, in which the signal at each point in the field of view is the result of a …
spatial cross-talk, in which the signal at each point in the field of view is the result of a …
Engineering the future of 3D pathology
JTC Liu, SSL Chow, R Colling… - The Journal of …, 2024 - Wiley Online Library
In recent years, technological advances in tissue preparation, high‐throughput volumetric
microscopy, and computational infrastructure have enabled rapid developments in …
microscopy, and computational infrastructure have enabled rapid developments in …
Expansion microscopy of apicomplexan parasites
Apicomplexan parasites comprise significant pathogens of humans, livestock and wildlife,
but also represent a diverse group of eukaryotes with interesting and unique cell biology …
but also represent a diverse group of eukaryotes with interesting and unique cell biology …