ONTbarcoder and MinION barcodes aid biodiversity discovery and identification by everyone, for everyone

A Srivathsan, L Lee, K Katoh, E Hartop, SN Kutty… - BMC biology, 2021 - Springer
Background DNA barcodes are a useful tool for discovering, understanding, and monitoring
biodiversity which are critical tasks at a time of rapid biodiversity loss. However, widespread …

$1 DNA barcodes for reconstructing complex phenomes and finding rare species in specimen‐rich samples

R Meier, W Wong, A Srivathsan, M Foo - Cladistics, 2016 - Wiley Online Library
Several of the biggest challenges in taxonomy and systematics are related to a toxic mixture
of small size, abundance, and rarity. There are too many species in groups with too few …

A DNA barcode library for 5,200 German flies and midges (Insecta: Diptera) and its implications for metabarcoding‐based biomonitoring

J Morinière, M Balke, D Doczkal… - Molecular ecology …, 2019 - Wiley Online Library
This study summarizes results of a DNA barcoding campaign on German Diptera, involving
analysis of 45,040 specimens. The resultant DNA barcode library includes records for 2,453 …

Exploring genetic divergence in a species-rich insect genus using 2790 DNA barcodes

X Lin, E Stur, T Ekrem - PloS one, 2015 - journals.plos.org
DNA barcoding using a fragment of the mitochondrial cytochrome c oxidase subunit 1 gene
(COI) has proven to be successful for species-level identification in many animal groups …

DNA barcoding of Chironomidae from the Lake Skadar region: Reference library and a comparative analysis of the European fauna

P Gadawski, M Montagna, B Rossaro… - Diversity and …, 2022 - Wiley Online Library
Aims The main aim of this study was to fill a gap in barcoding data of the European
Chironomidae of the Balkan region, developing and testing the efficiency of a reference …

ONTbarcoder 2.0: rapid species discovery and identification with real‐time barcoding facilitated by Oxford Nanopore R10. 4

A Srivathsan, V Feng, D Suárez, B Emerson… - Cladistics, 2024 - Wiley Online Library
Most arthropod species are undescribed and hidden in specimen‐rich samples that are
difficult to sort to species using morphological characters. For such samples, sorting to …

'Direct PCR'optimization yields a rapid, cost‐effective, nondestructive and efficient method for obtaining DNA barcodes without DNA extraction

WH Wong, YC Tay, J Puniamoorthy… - Molecular Ecology …, 2014 - Wiley Online Library
Macroinvertebrates that are collected in large numbers pose major problems in basic and
applied biodiversity research: identification to species via morphology is often difficult, slow …

Facing the infinity: tackling large samples of challenging Chironomidae (Diptera) with an integrative approach

C Chimeno, B Rulik, A Manfrin, G Kalinkat, F Hölker… - PeerJ, 2023 - peerj.com
Background Integrative taxonomy is becoming ever more significant in biodiversity research
as scientists are tackling increasingly taxonomically challenging groups. Implementing a …

NGS barcoding reveals high resistance of a hyperdiverse chironomid (Diptera) swamp fauna against invasion from adjacent freshwater reservoirs

B Baloğlu, E Clews, R Meier - Frontiers in Zoology, 2018 - Springer
Background Macroinvertebrates such as non-biting midges (Chironomidae: Diptera) are
important components of freshwater ecosystems. However, they are often neglected in …

MinION barcodes: biodiversity discovery and identification by everyone, for everyone

A Srivathsan, L Lee, K Katoh, E Hartop, SN Kutty… - BioRxiv, 2021 - biorxiv.org
DNA barcodes are a useful tool for discovering, understanding, and monitoring biodiversity.
This is critical at a time when biodiversity loss is a major problem for many countries …