Comparative evaluation of recombinant protein production in different biofactories: the green perspective

M Merlin, E Gecchele, S Capaldi… - BioMed research …, 2014 - Wiley Online Library
In recent years, the production of recombinant pharmaceutical proteins in heterologous
systems has increased significantly. Most applications involve complex proteins and …

Broadly neutralizing antibodies targeting HIV: Progress and challenges

N Paneerselvam, A Khan, BR Lawson - Clinical Immunology, 2023 - Elsevier
Anti-HIV broadly neutralizing antibodies (bNAbs) offer a novel approach to treating,
preventing, or curing HIV. Pre-clinical models and clinical trials involving the passive transfer …

Virologic effects of broadly neutralizing antibody VRC01 administration during chronic HIV-1 infection

RM Lynch, E Boritz, EE Coates, A DeZure… - Science translational …, 2015 - science.org
Passive immunization with HIV-1–neutralizing monoclonal antibodies (mAbs) is being
considered for prevention and treatment of HIV-1 infection. As therapeutic agents, mAbs …

Safety, pharmacokinetics and neutralization of the broadly neutralizing HIV-1 human monoclonal antibody VRC01 in healthy adults

Clinical & Experimental Immunology, 2015 - academic.oup.com
Summary VRC-HIVMAB060-00-AB (VRC01) is a broadly neutralizing HIV-1 monoclonal
antibody (mAb) isolated from the B cells of an HIV-infected patient. It is directed against the …

Delay of HIV-1 rebound after cessation of antiretroviral therapy through passive transfer of human neutralizing antibodies

A Trkola, H Kuster, P Rusert, B Joos, M Fischer… - Nature medicine, 2005 - nature.com
To determine the protective potential of the humoral immune response against HIV-1 in vivo
we evaluated the potency of three neutralizing antibodies (2G12, 2F5 and 4E10) in …

Engineering of Pichia pastoris for improved production of antibody fragments

B Gasser, M Maurer, J Gach, R Kunert… - Biotechnology and …, 2006 - Wiley Online Library
The methylotrophic yeast Pichia pastoris has been used for the expression of many proteins,
including antibody fragments. However, limitations became obvious especially when …

From gene to harvest: insights into upstream process development for the GMP production of a monoclonal antibody in transgenic tobacco plants

M Sack, T Rademacher, H Spiegel… - Plant biotechnology …, 2015 - Wiley Online Library
Summary The EU Sixth Framework Programme Integrated Project 'Pharma‐
Planta'developed an approved manufacturing process for recombinant plant‐made …

Broadly neutralizing antibodies for HIV eradication

KE Stephenson, DH Barouch - Current Hiv/aids Reports, 2016 - Springer
Passive transfer of antibodies has long been considered a potential treatment modality for
infectious diseases, including HIV. Early efforts to use antibodies to suppress HIV …

Antibody-mediated prevention and treatment of HIV-1 infection

H Gruell, F Klein - Retrovirology, 2018 - Springer
Novel broadly neutralizing antibodies targeting HIV-1 hold promise for their use in the
prevention and treatment of HIV-1 infection. Pre-clinical results have encouraged the …

Cost-effective production of a vaginal protein microbicide to prevent HIV transmission

K Ramessar, T Rademacher, M Sack… - Proceedings of the …, 2008 - National Acad Sciences
A series of small-molecule microbicides has been developed for vaginal delivery to prevent
heterosexual HIV transmission, but results from human clinical trials have been …