Over the past decade, CRISPR has become as much a verb as it is an acronym,
transforming biomedical research and providing entirely new approaches for dissecting all …

The development of new CRISPR–Cas genome editing tools continues to drive major
advances in the life sciences. Four classes of CRISPR–Cas-derived genome editing agents …

Genetic risk for autism spectrum disorder (ASD) is associated with hundreds of genes
spanning a wide range of biological functions,,,,–. The alterations in the human brain …

Fast and selective isolation of single cells with unique spatial and morphological traits
remains a technical challenge. Here, we address this by establishing high-speed image …

Identification of host genes essential for severe acute respiratory syndrome coronavirus 2
(SARS-CoV-2) infection may reveal novel therapeutic targets and inform our understanding …

Prime editing is a versatile genome editing tool but requires experimental optimization of the
prime editing guide RNA (pegRNA) to achieve high editing efficiency. Here we conducted a …

The immune system can eliminate tumors, but checkpoints enable immune escape. Here,
we identify immune evasion mechanisms using genome-scale in vivo CRISPR screens …

Epigenetic dysregulation is a defining feature of tumorigenesis that is implicated in immune
escape,. Here, to identify factors that modulate the immune sensitivity of cancer cells, we …

A plasmid Editor (ApE) is a free, multi-platform application for visualizing, designing, and
presenting biologically relevant DNA sequences. ApE provides a flexible framework for …

Gene editing that makes target gene modification in the genome by deletion or addition has
revolutionized the era of biomedicine. Clustered regularly interspaced short palindromic …