[PDF][PDF] 三维表面形貌测量中的共聚焦显微成像技术研究进展

王玥颖, 刘旭, 郝翔 - Laser & Optoelectronics Progress, 2023 - researching.cn
摘要随着精密仪器制造和半导体加工产业的蓬勃发展, 对微小结构表面形貌的观察和测量是现代
科学研究的一个重要方向. 激光扫描共聚焦显微成像技术因高分辨率, 高信噪比和优秀的层切 …

Bessel-beam illumination Raman microscopy

K Bando, S Yabuuchi, M Li, T Kubo… - Biomedical Optics …, 2022 - opg.optica.org
We demonstrate the use of Bessel beams for side illumination slit-scanning Raman imaging
for label-free and hyperspectral analysis of cell spheroids. The background elimination by …

Cryo-fluorescence micro-optical sectioning tomography for volumetric imaging of various whole organs with subcellular resolution

L Deng, J Chen, Y Li, Y Han, G Fan, J Yang, D Cao… - Iscience, 2022 - cell.com
Optical visualization of complex microstructures in the entire organ is essential for
biomedical research. However, the existing methods fail to accurately acquire the detailed …

A novel variational approach for multiphoton microscopy image restoration: from PSF estimation to 3D deconvolution

J Ajdenbaum, E Chouzenoux, C Lefort, S Martin… - Inverse …, 2024 - iopscience.iop.org
In multi-photon microscopy (MPM), a recent in-vivo fluorescence microscopy system, the
task of image restoration can be decomposed into two interlinked inverse problems: firstly …

Light microscopy at maximal precision

M Bierbaum, BD Leahy, AA Alemi, I Cohen, JP Sethna - Physical Review X, 2017 - APS
Microscopy is the workhorse of the physical and life sciences, producing crisp images of
everything from atoms to cells well beyond the capabilities of the human eye. However, the …

Rapid imaging of large tissues using high-resolution stage-scanning microscopy

T Yang, T Zheng, Z Shang, X Wang, X Lv… - Biomedical optics …, 2015 - opg.optica.org
Rapid and high-resolution imaging of large tissues is essential in biological research, like
brain neuron connectivity research and cancer margins imaging. Here a novel stage …

A deconvolution method to improve automated 3D-analysis of dendritic spines: application to a mouse model of Huntington's disease

N Heck, S Betuing, P Vanhoutte, J Caboche - Brain Structure and Function, 2012 - Springer
Dendritic spines are postsynaptic structures the morphology of which correlates with the
strength of synaptic efficacy. Measurements of spine density and spine morphology are …

A line scanning confocal fluorescent microscope using a CMOS rolling shutter as an adjustable aperture

E Mei, PA Fomitchov, R Graves… - Journal of …, 2012 - Wiley Online Library
Traditional confocal microscopy uses a physical aperture barrier to prevent out‐of‐focus
light from reaching the detector. The physical nature of a conventional aperture limits control …

Quantitative model of the image of a radiating dipole through a microscope

S Khadir, PC Chaumet, G Baffou, A Sentenac - JOSA A, 2019 - opg.optica.org
In this paper, we introduce a formalism to determine the relationship between the full
vectorial electric field existing at the object plane of a microscope and that existing at the …

International test results for objective lens quality, resolution, spectral accuracy and spectral separation for confocal laser scanning microscopes

RW Cole, M Thibault, CJ Bayles… - Microscopy and …, 2013 - academic.oup.com
As part of an ongoing effort to increase image reproducibility and fidelity in addition to
improving cross-instrument consistency, we have proposed using four separate instrument …