[HTML][HTML] The road to fully programmable protein catalysis
The ability to design efficient enzymes from scratch would have a profound effect on
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …
chemistry, biotechnology and medicine. Rapid progress in protein engineering over the past …
Designing artificial metalloenzymes by tuning of the environment beyond the primary coordination sphere
Metalloenzymes catalyze a variety of reactions using a limited number of natural amino
acids and metallocofactors. Therefore, the environment beyond the primary coordination …
acids and metallocofactors. Therefore, the environment beyond the primary coordination …
Conditionally activatable photoredox catalysis in living systems
The transformational effect of photoredox catalytic chemistries has inspired new
opportunities, enabling us to interrogate nature in ways that are not possible otherwise and …
opportunities, enabling us to interrogate nature in ways that are not possible otherwise and …
Artificial metalloenzymes: challenges and opportunities
Artificial metalloenzymes (ArMs) result from the incorporation of an abiotic metal cofactor
within a protein scaffold. From the earliest techniques of transition metals adsorbed on silk …
within a protein scaffold. From the earliest techniques of transition metals adsorbed on silk …
Building enzymes through design and evolution
EJ Hossack, FJ Hardy, AP Green - ACS Catalysis, 2023 - ACS Publications
Designing efficient enzymes is a formidable challenge at the forefront of modern
biocatalysis. Here, we review recent developments in the field and illustrate how the …
biocatalysis. Here, we review recent developments in the field and illustrate how the …
Design of heme enzymes with a tunable substrate binding pocket adjacent to an open metal coordination site
The catalytic versatility of pentacoordinated iron is highlighted by the broad range of natural
and engineered activities of heme enzymes such as cytochrome P450s, which position a …
and engineered activities of heme enzymes such as cytochrome P450s, which position a …
[HTML][HTML] Engineering and emerging applications of artificial metalloenzymes with whole cells
The field of artificial metalloenzymes (ArMs) is rapidly growing and ArMs are attracting
increasing attention, for example, in the fields of biosensing and drug therapy. Protein …
increasing attention, for example, in the fields of biosensing and drug therapy. Protein …
Synthetic prodrug design enables biocatalytic activation in mice to elicit tumor growth suppression
Considering the intrinsic toxicities of transition metals, their incorporation into drug therapies
must operate at minimal amounts while ensuring adequate catalytic activity within complex …
must operate at minimal amounts while ensuring adequate catalytic activity within complex …
Artificial metalloenzymes based on the biotin–streptavidin technology: enzymatic cascades and directed evolution
AD Liang, J Serrano-Plana, RL Peterson… - Accounts of chemical …, 2019 - ACS Publications
Conspectus Artificial metalloenzymes (ArMs) result from anchoring a metal-containing
moiety within a macromolecular scaffold (protein or oligonucleotide). The resulting hybrid …
moiety within a macromolecular scaffold (protein or oligonucleotide). The resulting hybrid …
[HTML][HTML] Biocompatibility and therapeutic potential of glycosylated albumin artificial metalloenzymes
The ability of natural metalloproteins to prevent inactivation of their metal cofactors by
biological metabolites, such as glutathione, is an area that has been largely ignored in the …
biological metabolites, such as glutathione, is an area that has been largely ignored in the …