CRISPR screens are a powerful source of biological discovery, enabling the unbiased
interrogation of gene function in a wide range of applications and species. In pooled …

It has been 10 years since the introduction of modern transposon-insertion sequencing (TIS)
methods, which combine genome-wide transposon mutagenesis with high-throughput …

High-throughput CRISPR-Cas9 screens have recently emerged as powerful tools to
decipher gene functions and genetic interactions. Here we use a genome-wide library of …

Abstract Development of hyperproducing strains is important for biomanufacturing of
biochemicals and biofuels but requires extensive efforts to engineer cellular metabolism and …

Many bacterial species are represented by a pan-genome, whose genetic repertoire far
outstrips that of any single bacterial genome. Here we investigate how a bacterial pan …

The vast majority of bacteria, including human pathogens and microbiome species, lack
genetic tools needed to systematically associate genes with phenotypes. This is the major …

High-throughput genetic screens are powerful methods to identify genes linked to a given
phenotype. The catalytic null mutant of the Cas9 RNA-guided nuclease (dCas9) can be …

The fast-growing Gram-negative bacterium Vibrio natriegens is an attractive microbial
system for molecular biology and biotechnology due to its remarkably short generation time …

Bacteriophages (phages) are critical players in the dynamics and function of microbial
communities and drive processes as diverse as global biogeochemical cycles and human …

Non-model microorganisms often possess complex phenotypes that could be important for
the future of biofuel and chemical production. They have received significant interest the last …